【作者】 徐春苗； 汤曼； 冯姣； 庞代文； 张志凌；

【Author】 Chun-Miao Xu;Man Tang;Jiao Feng;Dai-Wen Pang;Zhi-Ling Zhang;Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University;

【机构】 生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院；

【摘要】 循环肿瘤细胞(CTCs)是癌症的重要标志物之一,它的灵敏可靠检测对于癌症的早期诊断、预后评估和复发监测等具有重要的意义。但由于CTCs在血液中的含量少及其异质性,对它的检测仍存在挑战。由于磁场良好操控性能及易于与其他技术手段结合,磁控微流控芯片在CTCs等物质的分离和检测中应用广泛。这里,我们通过构建基于核酸适配体的磁控微流控芯片用于循环肿瘤细胞的高效检测。将磁性纳米颗粒与针对CTC表面的标志性蛋白的核酸适配体相连形成磁球-核酸适配体复合物(MNs-apt),然后把MNs-apt通入微流控芯片中形成MNs-apt阵列用于CTCs捕获。捕获的CTCs可在撤去磁场或核酸酶作用下释放用于后续分析研究。基于此实现CTCs的实时检测和有效释放用于后续分析,为癌症的早期诊断提供有效手段。更多还原

【Abstract】 Circulating tumor cells(CTCs) are one of the important biomarkers of cancer, its sensitive and reliable detection is important for early diagnosis, prognosis evaluation and recurrence monitoring of cancer. However, the low content and heterogeneity of CTCs limits its detection. Magnetic controlled microfluidic chip is widely used in separation and detection of CTCs and others, due to its good controllability and easy integration with other technologies. Herein, we constructed a magnetic chip based on aptamer detection CTCs efficiently. The aptamers for CTC were modified on magnetic nanospheres(MNs) to form MNs-apt, then the MNs-apt were introduced into the chip to form MNs-apt array for CTCs capture. The captured CTCs can be released for subsequent analysis by removing magnetic fields or nucleases. Based on this, the real-time detection and effective release of CTCs can be used for subsequent analysis and provide an effective means for the early diagnosis of cancer.更多还原