【作者】 杜永刚； 唐君； 崔勐； 刘志强； 刘淑莹；

【Author】 Yonggang Du;J.Tang;M.Cui;Z.Liu;S.Liu;Changchun Institute of Applied Chemistry,Chinese Academy of Sciences;

【机构】 中国科学院长春应用化学研究所；

【摘要】 人参,作为一种名贵的中药,被广泛的地应用于疾病的预防和治疗的方剂中。人参皂苷是人参中的主要活性成分之一,有着广泛的药理活性,如抗氧化、免疫调节等。研究人参皂苷和蛋白质的相互作用不仅有助于认识人参皂苷在人体内的吸收,转运,代谢等过程,而且这些相互作用对于揭示人参的药理过程有重要的意义。质谱以其灵敏度高,分析速度快,可以提供计量比信息等优点,在研究蛋白质与小分子配体之间的相互作用中有着广泛的应用。本文构建了基于质谱技术的人参皂苷与蛋白质相互作用的液相分析平台,可以很好的甄别人参皂苷与蛋白质的特异性非共价相互作用,方法简便、快捷。更多还原

【Abstract】 Ginseng as one of natural products has been widely used in traditional Chinese medicines. It has been found that ginsenosides are the main bioactive components in ginseng. The studies of interaction of ginsenosides with proteins will be helpful for investigation of metabolite process of ginsenosides, but also understanding of the biological activities of ginseng. Mass spectrometry has been applied in the investigation of interaction of protein and ligands with rapid analysis speed, high sensitivity and stoichmetry information. Here, based on mass spectrometry, the on-line analysis of interaction of ginsenosides and proteins has been developed. This strategy can distinguish the specific non-covalent interaction of ginsenosides and proteins.更多还原