【作者】 崔利利； 魏忠林； 郇延富； 李红梅； 费强； 李明；

【Author】 Lili Cui;Z.L.Wei;Y.F.Huan;H.M.Li;Q.Fei;M.Li;College of Chemistry, Jilin University;Department of Chemistry, National Institute of Metrology;

【机构】 吉林大学化学学院； 中国计量科学研究院化学计量与分析科学研究所；

【摘要】 电化学-质谱联用技术(EC-MS)作为一种新型的检测手段,在电化学反应产物和中间体的鉴定、蛋白质组学、药物代谢等方面有着广泛应用。这项工作主要使用电喷雾针有接地设计的ESI作为EC-MS的接口,研究了氧化型谷胱甘肽的还原和还原型谷胱甘肽的氧化行为。实验结果表明,该装置在检测电化学氧化还原行为有着灵敏、直观,高效、快速等优点。与之前的EC-MS相比,不仅实现仪器结构简单化,而且避免了对恒电位器件的损坏。更多还原

【Abstract】 As a new detection method, coupling electrochemical with mass spectrometry(EC-MS) has been widely used in the identification of electrochemical reaction products and intermediates, proteomics and drug metabolism. This work mainly introduced an alternative ESI with a grounded needle design as an interface of EC-MS and verified the availability of the device for the peptide through the reduction of oxidized glutathione and the oxidation of reduced glutathione. The results showed that the device had the advantages of sensitivity, intuition, efficiency and rapidness during the detection of electrochemical redox. Compared with the previous EC-MS, it not only simplifies the structure of the instrumentation, but also avoids damage to the electronic components of the potentiostat.更多还原