【作者】 陈淼淼； 陈月月； 赵嫚嫚； 左小双； 李响； 武海；

【Author】 Miaomiao Chen;Yueyue Chen;Manman Zhao;Xiaoshuang Zuo;Xiang Li;Hai Wu;School of Chemistry and Materials Engineering, Fuyang Normal University;

【机构】 阜阳师范学院；

【摘要】 纳米金刚石(ND)是一种典型的碳纳米材料,具有独特的生物兼容性和物理化学特性,而被广泛应用于药物投递和传感器领域。我们利用荧光光谱法研究了均匀分散的金刚石溶液与牛血清白蛋白(BSA)的相互作用,ND对BSA具有较强的荧光猝灭作用(图1)。根据荧光猝灭数据,研究了猝灭机理、键合常数及热力学参数(焓变、熵变和吉布斯自由能),猝灭机理为疏水作用力驱动下的自发的静态猝灭过程。另外,我们研究了ND及ND-BSA对人肺上皮细胞(BEAS-2B)的毒性,阐明了在无血清培养液中,低浓度ND毒性较小,高浓度ND则具有明显的细胞毒性。而ND-BSA的毒性明显弱于ND,说明BSA的引入可以在很大程度上抑制ND的毒性,为后续在分子水平上了解机体内蛋白质抵抗碳纳米材料对人体的危害提供了一定的依据。更多还原

【Abstract】 Nanodiamonds(NDs), a kind of carbon nanomaterials, possess special advantages of biocompatibility, physical, and chemical properties, which were intensively used in the fields of drug delivery vehicles and nanoscal sensors. The interaction mechanism between NDs and bovine serum albumin(BSA) has been studied by fluorescence spectroscopy. The results showed that NDs have strong fluorescence quenching effect on BSA and the quenching mechanism is a static quenching process. In addition, the cytotoxicity of NDs and ND-BSA to human lung epithelial cell line(BEAS-2B) was investigated, which showed that NDs exhibited cytotoxicity to BEAS-2B at high concentration while the cytotoxicity could be inhibited by the interaction with BSA.更多还原