【作者】 王艳伟； 周小娟； 许春丽； 金燕； 李保新；

【Author】 Yanwei Wang;Xiaojuan Zhou;Chunli Xu;Yan Jin;Baoxin Li;School of Chemistry & Chemical Engineering, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Shaanxi Normal University;

【机构】 陕西省生命分析化学重点实验室陕西师范大学化学化工学院；

【摘要】 本文以谷氨酰胺为模型氨基酸,研究了金纳米棒对氨基酸的手性识别性能。实验发现D-谷氨酰胺可以使金纳米棒发生团聚,溶液的颜色由蓝色变为灰色,而L-型谷氨酰胺不能使金纳米棒团聚,溶液颜色不变。此外,将谷氨酰胺外消旋体加到金纳米棒溶液中,当D-谷氨酰胺的ee值从-100%增加到100%时,金纳米棒溶液的颜色呈现规律性变化,可根据金纳米棒的紫外可见吸收光谱检测谷氨酰胺的光学纯度。该体系还可以识别多种氨基酸对映体。本文通过多种手段探究了金纳米棒手性识别谷氨酰胺的机理。本研究使用的金纳米棒无需手性修饰,具有简单、快速、低成本等优点,且可高通量识别多种氨基酸对映体。更多还原

【Abstract】 In this sensing platform, gold nanorods(Au NRs) are used as colorimetric probes for visual recognition of glutamine(Gln) enantiomers. D-Gln could rapidly induce the aggregation of AuNRs, thereby resulting in appreciable blue-to-gray color change of AuNRs solution; however, L-Gln could not induce color change of AuNRs. This distinct color change can be easily distinguished by the naked eyes; as a result, a visual method of chiral recognition was suggested. The method was applied to determine the enantiometric excess of D-Gln through the whole range of-100% ～ 100%.Notably, the AuNRs do not need any chiral labeling or modification, and the chiral recognition is based on the inherent chirality of AuNRs. This chiral assay method is simple, sensitive, cheap and easy to operate. This study is the first example using AuNRs for direct visual recognition of enantiomers, and will open new opportunity to construct more chiral recognition methods for some important compounds.更多还原