【作者】 王顺； 李伟； 司思思； 郭清乾； 张浩； 胡建东；

【Author】 Shun Wang;Wei Li;Sisi Si;Qingqian Guo;Hao Zhang;Jiandong Hu;College of Mechanical and Electrical Engineering, Henan Agricultural University;College of Science, Henan Agricultural University;

【机构】 河南农业大学机电工程学院； 河南农业大学理学院；

【摘要】 脱落酸作为植物生长过程中的必要植物激素之一,具有促进植物生长,维持植物幼芽与种子休眠,增强植物的抗逆等作用。随着植物激素调控机理和信号转导等前沿领域的兴起,迫切需要实现脱落酸快速、高灵敏检测。本研究基于贵金属纳米颗粒特有的局域表面等离子共振(LSPR)性质,即共振波长与介电环境、纳米颗粒形貌、尺寸等因素密切相关,进行脱落酸生物传感检测。论文首先搭建了一套由宽带光源、衰减器、光纤光谱仪、多通道样品池精准定位机构等组成的光谱实验装置,然后根据目标物加入前后金纳米粒子形貌变化引起的LSPR波长漂移,可以实现脱落酸的定量检测,最低检测浓度可低至1 nM。实验中采用了光纤光谱仪,无需光谱扫描过程,2 min内即可完成6个样品的检测,从而实现了脱落酸快速、高灵敏检测。更多还原

【Abstract】 Abscisic acid(ABA) plays an important role in abiotic stress response and physiological signal transduction resisting to the adverse environment. Therefore, it is very essential for the quantitative detection of ABA due to its indispensable role in plant physiological activities. The localized surface plasmon resonance(LSPR) technique is widely used in the field of biosensing. A multi-channel LSPR measurement system was constructed by utilizing a broadband light source, a light attenuator, an optical fiber spectrometer and a multi-channel sample cell customized with a precision alignment mechanism. No spectral scanning process was required due to the use of optical fiber spectrometer. According to the changes of LSPR peak wavelength of AuNPs, six samples can be completely detected within two minutes, and the minimum detection concentration of ABA can be reduced to 1 nM.更多还原