【作者】 刘颖； 任克维； 张玥； 何玉玲； 鞠熀先；

【Author】 Ying Liu;KW Ren;Y Zhang;YL He;HX Ju;Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University;

【机构】 南京大学生命分析化学国家重点实验室化学化工学院南京大学；

【摘要】 细胞内生物标志物的精准成像与高效基因干扰在当前癌症与其它疾病的治疗中发挥着重要作用,其中保护小分子探针在复杂生物环境的递运过程中不被降解并实现靶点上的高效率、高选择性的可控释放是富有挑战性的问题。我们设计了一系列DNA自组装纳米材料与可降解高分子包裹的上转换纳米材料,并将其作为成像探针与小干扰RNA的递运载体,通过包括近红外光照[1]、细胞膜上受体选择[2]等外部刺激与活性氧、细胞内m RNA[3]等内部刺激实现了细胞内的可控递运及高效成像与治疗。这些刺激响应型纳米材料载体具有大比表面积、刚性结构与良好的生物相容性,能够通过局域空间限制反应物扩散而增加反应效率并通过与细胞膜上不同受体的连续反应增加反应特异性,从而表现出体外与体内良好的成像与治疗效果,在精准医疗中具有应用前景。更多还原

【Abstract】 Precise imaging of intracellular biomarker and highly efficient RNA interference(RNAi) have become appealing therapeutic approaches for cancer and other diseases nowadays. One key challenge is the effective protection of the small fragile biomolecules agents against complicated physiological environment as well as on-demond intracellular release with high efficiency and selectivity. Here we designe self-assembled DNA nanomaterial and degradable polymer conjugated upconversion nanoparticle(UCNP) as delivery carriers for imaging agents and therapeutic si RNA, while both external stimulus, such as light and cell membrane receptors, as well as internal stimulus, such as radical oxygen species(ROS) and m RNA, are applied to activate the cargoes and achieve intracellular imaging and therapy. The nanostructured delivery carriers have large surface areas with rigid structure and good biocompatibility, which provide high reaction efficiency and impressive delivery specificity. These designed stimulus-responsive nanomaterials provide good imgaing and therapeutic efficiencies, therefore have potential application in precision medicine.更多还原