【作者】 王瑾； 字琴江； 赵文颖； 周川华； 曹秋娥；

【Author】 Jin Wang;Qin-Jiang Zi;Wen-Ying Zhao;Chuan-Hua Zhou;Qiu-E Cao;School of Chemical Science and Technology, Yunnan University;

【机构】 云南大学化学科学与工程学院；

【摘要】 将免疫磁分离、酶促金属化信号放大策略与液晶生物传感相结合,建立了一种抗干扰能力强的液晶免疫传感器并将其应用于肿瘤标志物的高灵敏检测。我们采用磁球作为免疫反应的载体[1],利用生物素化的抗体和链霉亲和素标记的葡萄糖氧化酶（SA-GOD）作为信号标记物,在磁球表面形成磁性免疫夹心复合物。然后通过GOD的催化作用诱导银沉积[2]到修饰了纳米金的玻片基底表面,利用其对液晶分子有序排列的扰乱来实现信号放大。随着CEA的浓度增加,偏光显微镜观察到的图像上的亮点逐渐增多并逐渐转变为不同颜色的织构图像（Fig. 1）。采用该方法能够在1×10^-7～1×10^-13 g/mL范围内实现对CEA的可视化检测,检出限低至1×10^-13 g/mL。该方法解决了液晶生物传感器应用于免疫分析时灵敏度不高、抗干扰能力不足的问题,可以实现复杂样品中肿瘤标志物的高灵敏可视化检测。更多还原

【Abstract】 A liquid crystal immunosensor with strong anti-interference ability was established by integrating immunomagnetic separation and enzyme-induced metallization amplification with liquid crystal biosensor, which was further applied to tumor marker detection with high sensitivity. The immunomagnetic beads were used as the reaction carrier, the biotinylated antibody and streptavidin labeled glucose oxidase（SA-GOD） were used as the signal tags. The presence of glucose oxidase（GOD） on the surface of the immunocomplex-coated magnetic beads could induce silver deposited on the Au nanoparticles modified substrate, which can disarrange the ordered arrangement of liquid crystal molecules and thus amplify the detection signal. Under the optimal conditions, CEA could be detected in the range of 1×10^-7～1×10^-13 g/mL. The proposed method has the advantages of high-sensitivity,strong anti-interference ability and simple instruments, which could be applied to complex samples.更多还原