【作者】 陈子晗； 韩欣悦； 施国跃； 张闽；

【Author】 Zi-Han Chen;Xin-Yue Han;Guoyue Shi;Min Zhang;School of Chemistry and Molecular Engineering, East China Normal University;

【机构】 华东师范大学化学与分子工程学院；

【摘要】 本工作中我们使用简便的水热法制备了Tb³⁺掺杂的碳点(Tb³⁺@CDs),并设计了一段富含GT碱基的单链DNA（ss DNA）与之形成Tb³⁺CDs/ssDNA的复合物。通过加入Ag⁺和半胱氨酸（Cys）能够可逆调控该复合物的比率荧光,即:Tb³⁺在546 nm处的荧光峰作为信号指示剂,而444 nm处碳点的荧光峰作为内标保持不变。基于Ag⁺/Cys介导的Tb³⁺CDs/ssDNA可逆荧光变化我们设计了一种自校准的比率型荧光逻辑系统;进一步结合H₂O₂和Cys之间的特异性反应,该方法可以扩展到监测由氧化酶参与相关生化反应,例如葡萄糖氧化酶催化氧化葡萄糖。更多还原

【Abstract】 Tb³⁺-doped carbon dots (Tb³⁺@CDs) were prepared in a facile hydrothermal method.A 15-bp GT-rich single-strand DNA （ssDNA） was introduced to form a conjugate of Tb³⁺@CDs/ssDNA.The ratiometric fluorescence of Tb³⁺@CDs/ssDNA could be reversibly regulated by Ag⁺and Cys,in which the fluorescence peak at 546 nm of Tb³⁺could be switched to"On"or"Off"as the signal indicator while the fluorescence peak at 444 nm of CDs remained constant as the build-in reference.The proposed Ag⁺/Cys-mediated reversible fluorescence changes in Tb³⁺@CDs/ssDNA was proven for the design of a self-calibrating ratiometric fluorescence logic system.By integrated with the specific reaction between H₂O₂ and Cys,the sensing strategy can be further successfully extended to the monitoring of oxidase-related reactions,such as GOx-catalyzed oxidation of glucose.更多还原