【作者】 吴传丽； 梁文慧； 吴萍； 蔡称心；

【Author】 Chuanli Wu;Wenhui Liang;Ping Wu;Chenxin Cai;College of Chemistry and Materials Science, Nanjing Normal University;

【机构】 南京师范大学化学与材料科学学院；

【摘要】 蛋白质(酶)的电子传输(ETp)是指电子通过整体蛋白质的过程,它不仅在呼吸作用、光合作用、各种代谢循环过程中起重要作用1,而且与生物电子学、生物电催化及纳米生物技术等领域密切相关2-4,但如何实现蛋白质ETp并保持原有的构象仍是一个未解决的基础性问题。本工作研究了氯化血红素(hemin)对牛血清白蛋白(BSA)的掺杂以及掺杂后其对BSA构象及ETp特性的影响。首先,根据紫外-可见光谱和荧光光谱特征峰的变化,确定了BSA-hemin复合物的成功合成;其次,利用CD光谱技术表征BSA掺杂后的构象变化,发现hemin对BSA的构象几乎不产生影响;最后,运用电化学方法研究了hemin对BSA的ETp性能的影响,结果表明hemin可以显著提高BSA的ETp性能。更多还原

【Abstract】 Electron transport(ETp) in proteins(including enzymes) refers to such a process that electrons are carried across the entire protein molecule. It plays a key role in many biological processes, such as respiration, photosynthesis, and various metabolic cycles. Furthermore, ETp of proteins is also of great interest in the fields of bioelectrocatalysis, bioelectronics, and more recently, nanobiotechnologies. However, how to achieve, and further to enhance the ETp properties of proteins at their native conformations is an important fundamental issue, which is still remained to be unsolved. Here we chose to study bovine serum albumin(BSA) and tuning their ETp efficiency by doping them with hemin. The results indicated that binding of hemin to BSA increases remarkably the ETp efficiency of protein and retain their primary structure simultaneously.更多还原