【作者】 王振新； 田荣荣； 雷桢；

【Author】 Zhenxin Wang;Rongrong Tian;Zhen Lei;State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry Chinese Academy of Sciences;University of Science and Technology of China;

【机构】 中国科学院长春应用化学研究所电分析化学国家重点实验室； 中国科学技术大学应用化学与工程学院；

【摘要】 微阵列芯片技术因具有高通量、平行性、自动化和样品消耗量少等优点而被广泛应用于生命科学和分析检测等研究领域。我们在不同基底上构建了一系列以微阵列芯片为反应平台的荧光分析方法用于肿瘤相关蛋白酶活性分析和糖基化合物生物标志物筛选,为疾病的诊断和预后提供依据。使用所构建的基于多肽芯片的金属荧光增强法成功的实现了不同细胞分泌物及乳头状甲状腺癌(PTC)和甲状腺结节(TN)病人的临床组织等复杂样品中多种基质金属蛋白酶(Matrix metalloproteinases,MMPs)的活性分析。通过不同细胞株与含有20种凝集素的三维凝胶凝集素芯片相互作用,获得了一种能够与结直肠肿瘤SW480细胞特异性作用的凝集素(Uelx Europaeus Agglutinin I,UEA-I)。小动物多模态成像以及临床结直肠组织标记等实验结果表明UEA-I能够做为结直肠肿瘤SW480亚型的生物标记物。更多还原

【Abstract】 Due to the advantages of high-throughput, parallelism, automation and lower sample consumption, microarrays have been extensively used in the field of biological science and bioanalysis. We have fabricated a series of fluorescence assays using microarray as reaction platform on different substrates to analyze the activity of tumor related proteases and screening the glycan biomarkers of diseases. A peptide microarray-based metal enhanced fluorescence assay was applied to profile multiplex MMPs(Matrix metalloproteinases) activities in various cell lines and clinical thyroid tissue samples of papillary thyroid carcinoma(PTC) patients and thyroid nodules(TN) patients, and verified the relationship between MMPs and tumor invasion and metastasis. A polyacrylamide hydrogel-based lectin microarray was fabricated to screen new lectin biomarker for colorectal cancer. Uelx Europaeus Agglutinin I(UEA-I) has been demonstrated to have high affinity and specificity with SW480 cells. Furthermore, the UEA-I has been successfully applied to differentiate mouse-bearing SW480 CRC by multimodal imaging.更多还原