【作者】 刘凤平； 徐琴； 张贞发； 向刚； 张翠忠； 彭金云；

【Author】 Fengping Liu;Qin Xu;Zhenfa Zhang;Gang Xiang;Cuizhong Zhang;Jinyun Peng;Department of Chemistry and Biological Science, Guangxi Normal University for Nationalities;College of Chemistry and Chemical Engineering, Yangzhou University;

【机构】 广西民族师范学院； 扬州大学；

【摘要】 本文采用柠檬酸钠为还原剂制备多孔石墨烯。过氧化氢酶固定在多孔石墨烯上修饰玻碳电极,对H₂O₂进行检测发现,线性范围为1.0×10^-7～7.7×10^-6 M,检出限为83 nM.基于2,4-二氯苯氧乙酸（2,4-D）对过氧化氢酶活性抑制用于2,4-D的研究,结果表明线性范围为2.4×10^-9～3.6×10^-7M,检出限为1.5 nM,抑制常数为6.50×10^-8M,成功用于豆芽菜中2,4-D的检测。更多还原

【Abstract】 This work reported a facile and green method to prepare porous graphene （PGR） based on sodium citrate.A novel H₂O₂ biosensor was constructed by immobilizing catalase on the PGR.The biosensor shows a linear range from 1.0×10^-7～7.7×10^-6 M with a low detection limit of 83 nM for H₂O₂.In addition,the biosensor was also carried to measure 2,4-dichlorophenoxyacetic acid （2,4-D） based on the inhibition effect of 2,4-D to the activity of the immobilized catalase.The inhibition ratio was linearly proportional to the concentration of 2,4-D in the range of 2.4×10^-9～3.6×10^-7M,The detection limit was down to 1.5 nM.The inhibition constants （K_i） was calculated to be 6.50×10^-8M.The results of detecting 2,4-D in bean sprouts were satisfactory.更多还原