【作者】 赵若男； 贾丽萍； 王怀生；

【Author】 Ruonan Zhao;Liping Jia;Huaisheng Wang;Department of Chemistry, Liaocheng University;

【机构】 聊城大学；

【摘要】 MUC1粘蛋白（Mucin 1）,是一种Ⅰ型跨膜蛋白,由于其在肿瘤组织中的异常表达,已成为一种潜在的肿瘤生物学标志物,目前已用于肿瘤的诊断和生物学治疗。本工作制备了具有高催化活性的PtPd双金属纳米粒子,发现该PtPd NPs具有类过氧化物酶的性质,对H₂O₂氧化3, 3’, 5, 5’-四甲基联苯胺（TMB）的反应具有催化作用。基于此,以发夹DNA功能化的PtPd NPs为指示探针,通过引入巧妙设计的三条发夹DNA链（HP1, HP2,HP3）可以使目标物的循环利用,实现了信号放大。该传感器对Mucin1的检测具有灵敏度高、检测限低等优点,有望用于Mucin 1的临床检测。更多还原

【Abstract】 Protein biomarkers, as one important class of biomarkers, can be indicative of disease state according to their high or low expression in serum or tissue. In this paper, a general and facile way was developed to prepare PtPd bimetallic NPs, which exhibit peroxidase-like activity to the oxidative reaction between H₂O₂ and TMB. Herein, we fabricated a novel electrochemical aptasensor for ultrasensitive detection of Mucin 1 using hairpin DNA functionalized PtPdNPs as the indicative probe. The introduction of three ingenious design hairpin DNA strands（HP1, HP2 and HP3） achieved the cyclic reuse of target protein, which made the signal be amplified greatly. With excellent sensitivity and selectivity, the strategy may provide an efficient method for clinical application.更多还原