【作者】 姜玲； 刘松琴；

【Author】 Jiang Ling;Liu Songqin;Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University;

【机构】 东南大学化学化工学院；

【摘要】 细胞色素P450酶系在药物代谢过程中发挥着重要作用。通过体外模拟揭示CYP450酶对药物的代谢机理和化学本质,研究药物对酶的诱导和抑制效应,可为新药的设计和合成提供依据,对于人类健康具有重要的现实意义和实践价值。本实验利用具有分级多孔结构且比表面积较大的碳纳米笼材料为酶负载固相基质,在该纳米材料上通过静电吸附的方式组装不同类型的模型酶,构建具有生物催化功能的纳米反应器和生物传感器。结果表明,由于碳纳米笼具有较大孔径,特定的限域效应,优异的导电性以及良好的生物相容性,保证了较快的传质过程,进而提高了酶的催化反应速率。更多还原

【Abstract】 Cytochrome P450 enzymes(cyt P450s) are a superfamily of heme proteins, which are responsible for the oxidative metabolism of ～73% clinical drugs. Herein, an electrochemical platform for the investigation of drug metabolism in vitro was constructed by immobilizing cytochrome P450 2C9(CYP2C9) on carbon nanocages modified glass carbon electrode. Due to the hierarchically pore structure, large specific surface area, and good electrical conductivity of carbon nanocages, the electrochemical-driven drug Metabolism was realized. Furthermore, this system was applied to studying the metabolism of other drugs.更多还原