【作者】 王珊珊； 李海娟； 赵阳阳； 金永东； 葛春华；

【Author】 Shanshan Wang;Haijuan Li;Yangyang Zhao;Yongdong Jin;Chunhua Ge;State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry;Liaoning University;

【机构】 中国科学院长春应用化学研究所电分析化学国家重点实验室； 辽宁大学；

【摘要】 金属有机骨架（MOFs）因其多孔性及大的比表面积、结构和功能的多样性和不饱和金属位点被广泛关注与应用。在本实验的设计中,选用一种富含钴离子的MOFs,将其作为纳米载体,催化鲁米诺还原的Ag纳米粒子复合物（鲁米诺-AgNPs）,使其富集在MOFs表面,产生电化学发光。值得注意的是,复合后的MOFs@鲁米诺-AgNPs相比鲁米诺-AgNPs表现出极高的ECL性能,提高了两个数量级。通过构建无标记的自组装免疫传感器,检测心肌梗死的主要标志物心肌肌钙蛋白,表现出较好的选择性,同时检测限极低,可达到0.1pg·mL^-1。如此令人满意的性能,不仅拓宽了MOF的应用范围,同时为检测心肌梗死提供了一个很好的方式。更多还原

【Abstract】 Metal organic frameworks（MOFs） are widely concerned and applied due to their porosity and large specific surface area, diversity of structure and function, and unsaturated metal sites. In the design of this experiment, cobalt-ion-rich MOFs material was selected as nanocarriers to catalyze the capture of ECL reagent of luminol-reduced Ag nanoparticles（luminol-AgNPs） to enrich them on the surface of MOFs. It is worth noting that the compounded MOF@Luminol-AgNPs exhibited extremely highier ECL performance compared to Luminol-AgNPs, enhanced two orders of magnitude. Ultimately, a label-free ECL immunosensor was constructed for the detection of cardiac troponin（cTnI） which as the main marker of myocardial infarction. The immunosensor was detected with good selectivity and had extremely low detection limit of 0.1 pg·mL^-1. Such satisfactory performance can not only broaden the scope of application of MOF, but also provides a new way to detect myocardial infarction.更多还原