基于piggyBac转座子系统的西方蜜蜂Rubia基因转移研究

【作者】 柯俐； 胡小芬； 王子龙； 曾志将； 何旭江；

【机构】 江西农业大学蜜蜂研究所；

【摘要】 为探讨piggyBac载体在西方蜜蜂(Apis mellifera)中的基因转移功能,并检测其可行性,本研究以西方蜜蜂为实验材料,利用piggyBac转座子系统将外源性Rubia红色荧光蛋白基因转移至西方蜜蜂受精卵中,分析检测外源基因的整合与荧光表达情况。PCR扩增和测序分析结果显示piggyBac转座子能够将外源性Rubia红色荧光蛋白基因整合到蜜蜂基因组上,且在显微注射组中检测到微弱的红色荧光蛋白表达。本研究证实piggyBac转座子系统可将外源性基因植入蜜蜂基因组。这为今后的蜜蜂转基因基础研究和分子育种技术开发提供基础。更多还原

【Abstract】 To explore the gene transfer function of piggyBac transposon in western honeybees(Apis mellifera) and to further examine its feasibility,in this study,Apis mellifera was used as an experimental material.The piggyBac transposon system was used to transfer the exogenous red fluorescent protein gene Rubia into the honeybee zygotes,for the analysis and detection of foreign gene integration and fluorescence expression in honeybees.PCR amplification and sequencing analysis showed that the piggyBac transposon was able to integrate the exogenous Rubia red fluorescent protein gene into the honeybee genome.Fluorescence microscopy detection also showed a weak red fluorescent protein expression.This study confirmed that the piggyBac transposon system could implant exogenous gene into honeybee genome.This would provide a basis for the future development of honeybee transgenic research and exploitation in molecular breeding.更多还原