【作者】 吴小波； 周林斌； 邹垂彬； 颜伟玉； 曾志将；

【Author】 Wu Xiao-bo;Zhou Lin-bin;Zou Chui-bin;Yan Wei-yu;Zeng Zhi-jiang;Honeybee Research Institute,Jiangxi Agricultural University;

【机构】 江西农业大学蜜蜂研究所；

【摘要】 本试验利用蜜蜂仿生免移虫育王技术探索不同王台孔径(9.4mm、9.6mm、9.8mm与10.0mm)对意大利蜜蜂蜂王培育质量的影响,在此基础上进一步探索囚王时间(0 d、2 d与4 d)对意蜂蜜蜂蜂王培育质量的影响。结果表明:随着王台孔径的增加,其培育蜂王的初生重、卵巢管数、胸长与胸宽指标以及蜂王腹部的卵黄蛋白原基因(Vg)表达量均显著上升,这说明王台孔径显著影响培育蜂王质量及其繁殖能力;另外,囚禁蜂王的时间越长,其产下卵的卵重、卵长与卵宽均显著上升,而且其培育蜂王的初生重、卵巢管数、蜂王胸长与胸宽以及蜂王腹部的卵黄蛋白原基因(Vg)表达量也均显著上升,这也说明囚王时间明显影响蜂王所产卵的大小以及所培育的蜂王质量。更多还原

【Abstract】 This study aims to investigate the effect of queen cells with different diameters(9.4 mm、9.6 mm、9.8 mm、10.0 mm) on the quality of rearing queens using the queen rearing method without grafting larvae.On this basis,the effect of queen caging time(0 d、2 d and 4 d) on the quality of rearing queens Apis mellifera L with the best queen cell diameter were studied.Our results showed that the birth weight,ovarioles,thorax length and width were all significantly increased with the growth of queen cell diameters.qPCR results showed a similar trend that the expression of Vitellogenin(Vg) in queen abdomen were upregulated with the queen cell size increase.These results indicate that the queen cell size can strongly affect the queen quality and reproductive ability.Moreover,the egg weight,length and width raised with the queen caging time,and queens from these eggs were also increased in terms of birth weight,ovarioles,thorax length and width.Further,the expression of Vitellogenin(Vg) in queen abdomen were upregulated with the caging time.These results revealed that the queen caging time could significantly influence egg size and their relative queen quality.更多还原