【作者】 张艳平； 郭书贤； 王冬梅； 张志立； 李斌；

【Author】 GUO Shu-xian;WANG Dong-mei;ZHANG Yan-ping;ZHANG Zhi-li;LI Bin;Key Laboratory for Industrial Microbiology of Nanyang City;State Key Laboratory for Automotive Biofuel;School of Biological and Chemical Engineering, Nanyang Institute of Technology;

【机构】 南阳理工学院生物与化学工程学院； 2南阳市工业微生物重点实验室； 车用生物燃料技术国家重点实验室；

【摘要】 从河南省南阳地区土壤中分离的10株野生酵母菌株中,筛选到一株能够同步利用葡萄糖和木糖发酵高产油脂的酵母菌株ZZ-46。摇瓶发酵120 h,在葡萄糖:木糖为2:1条件下,生物量、油脂产量、油脂含量、油脂系数和产油速率分别达到20.23g/L、9.89 g/L、48.91%、14.64g/100g和0.083 g·L^-1·h^-1;该菌株利用5种不同比例混合糖（葡萄糖和木糖）产油脂的脂肪酸组成均以C16和C18系脂肪酸为主,其中油酸含量最高,其次为亚油酸、棕榈酸和硬脂酸,四种脂肪酸含量占总脂肪酸含量的90%以上,与植物油脂脂肪酸组成相似,可以作为生物柴油油源。通过形态特征及26S rDNA D1/D2区域序列分析,初步鉴定菌株ZZ-46为Cutaneotrichosporon dermatis,与Trichosporon dermatis同物异名。更多还原

【Abstract】 In this paper, the strain ZZ-46 which could assilimated glucose and xylose simultaneously producing lipid were screened from 10 wild yeast strains from the soil of Nanyang region of henan province. The cell biomass, lipid concentration, lipid content, lipid coefficient and lipid productivty of the strain reached 20.23 g/L、9.89 g/L、48.91%、14.64 g/100 g and 0.083 g·L^-1·h^-1 respectively by fermenting 120 h with 2: 1 of glucose and xylose as carbon source. Simultaneous, Lipid-acid component was analyzed, there is almost no difference in lipid-acids component by using different mixed sugar ratios as carbon source and C₁₆ and C₁₈ series are main fatty acids. Oleic acid content is the highest in lipid-acid component, next are linoleic acid, palmitic acid and stearic acid in all samples and the sum account for over 90%. The lipid component of this strain is similar to plant oil and could be used as biodiesel source. The strain was primarily identified as Cutaneotrichosporon dermatis by the morphology feature and 26 S rDNA D1/D2 domain sequence analysis, and Trichosporon dermatis homonym.更多还原