Construction of Histone H3K14A mutant of Saccharomyces Cerevisiae

【作者】 薛涛涛； Jin-Bian； 赵秀娟； Lu Cai；

【Author】 Tao-Tao Xue;Jin-Bian;Xiu-Juan Zhao;Lu Cai;School of Mathematics Physics and Biological Engineering,Inner Mongolia University of Science and Technology;

【机构】 内蒙古科技大学； School of Mathematics Physics and Biological Engineering,Inner Mongolia University of Science and Technology；

【摘要】 Background:Histone modification is one of the key epigenetic regulation mechanisms which plays a critical role in various biological processes.In Saccharomyces cerevisiae,the histones H3 and H4 gene are encoded by two copy,named HHT1-HHF1 and HHT2-HHF2.Specific sites of histone acetylation,methylation and other modifications have a important biological significance to eukaryotic DNA replication.Methods:We used the method of two-step substitution.First,we used gene knockout technology to replace HHT1-HHF1 gene on Chromosome Ⅱ with NatMX4 gene,then we knock out HHT2-HHF2 gene on ChromosomeⅩⅣ with the hhtS-HHFS mutant gene.Results:Histone H3 K14 A mutant strain in yeast BY4741 were obtained successfully.更多还原

【Abstract】 Background:Histone modification is one of the key epigenetic regulation mechanisms which plays a critical role in various biological processes.In Saccharomyces cerevisiae,the histones H3 and H4 gene are encoded by two copy,named HHT1-HHF1 and HHT2-HHF2.Specific sites of histone acetylation,methylation and other modifications have a important biological significance to eukaryotic DNA replication.Methods:We used the method of two-step substitution.First,we used gene knockout technology to replace HHT1-HHF1 gene on Chromosome Ⅱ with NatMX4 gene,then we knock out HHT2-HHF2 gene on ChromosomeⅩⅣ with the hhtS-HHFS mutant gene.Results:Histone H3 K14 A mutant strain in yeast BY4741 were obtained successfully.更多还原