【作者】 孙青； 段梦婷； 范伟； 范兵；

【Author】 Qing Sun;Mengting Duan;Wei Fan;Bing Fan;The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education,School & Hospital of Stomatology, Wuhan University;

【机构】 口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室武汉大学口腔医学院；

【摘要】 目的 :本研究采用模板法合成了银锌钙硅介孔纳米材料(MCSN-Ag-Zn),并观察其对根管再感染的优势菌-粪肠球菌的抑菌效果。方法 :采用FE-SEM、TEM、BET和ICP-AES表征MCSN-AgZn材料的理化性能,通过平板计数法观察MCSN-Ag-Zn抗粪肠球菌效果。结果 :球形颗粒直径约为100nm且具有牙本质小管渗透性;内部为多介孔结构(3.96±0.22 nm),比表面积高达543.56±44 m2/g,载药后未显著降低材料的比表面积;MCSN-Ag-Zn纳米材料对粪肠球菌具有良好的抑菌效果。结论:MCSN-Ag-Zn纳米材料具有良好的理化性能、生物相容性、抗菌能力及牙本质小管渗透能力,可能成为一种新型的根管内消毒药物。更多还原

【Abstract】 Objective: A silver-zinc-calcium-silicon mesoporous nanoparticles(MCSN-Ag-Zn) was synthesized using a template method, and its antibacterial effect on Enterococcus faecalis, a dominant bacterium that reinfects root canals, was observed. Methods: After synthesis of the new material MCSNAg-Zn with templated method derived from our previous study, we characterized the physico-chemical properties of nanoparticles using field emission-scanning electron microscopy(FE-SEM), transmission electron microscopy(TEM), Brunauer–Emmett–Teller and the Barrett–Joyner–Halenda analyses and inductively coupled plasma-atomic emission spectrometry(ICP-AES). The antibacterial ability of MCSNAg-Zn against planktonic E.faecalis was tested by counting colony-forming units(CFUs) method. Results: The nanoparticles of the MCSN-Ag-Zn were an approximate sphere with a size of about 100 nm and had abundant mesopores or channels inside whose size were 3.96±0.22 nm. Its specific surface area was 543.56±44 m2/g and not significantly reduced after loading silver and zinc. Moreover, MCSN-Ag-Zn nanomaterials have significant antibacterial effects against Enterococcus faecalis. Conclusions: MCSN-AgZn nanomaterials have favorable physical and chemical properties, biocompatibility, antibacterial ability and dentinal tubule penetration ability, and could be developed into a new intra-canal disinfectant for dental treatments.更多还原