【作者】 赵淑兰； 黄振兴； 阮文权； 缪恒锋； 赵明星；

【Author】 ZHAO Shulan;HUANG Zhenxing;RUAN Wenquan;School of Environmental and Civil Engineering,Jiangnan University;Jiangsu Key Laboratory of Anaerobic Biotechnology;

【机构】 江南大学环境与土木工程学院； 江苏省厌氧生物技术重点试验室；

【摘要】 餐厨垃圾中含有大量的有机碳源,具有资源化回收利用的潜力。本文以三相分离后的餐厨固渣为研究对象,采用酶法和和等电点沉淀法分离餐厨垃圾中的有机碳源。结果显示,α-淀粉酶和γ-淀粉酶的协同作用能够有效分离餐厨固渣中的碳源。单因素实验基础上采用Box-Behnken响应曲面法优化酶解关键参数,在温度57.6℃、pH值6.25、底物浓度20%（以TS计）、酶添加量0.8%、α-淀粉酶和γ-淀粉酶添加比3:1的最优条件下,碳源提取率高达76.9%。复合酶解过程符合Michaelis-Menten动力学模型,米氏常数Km为31.7 g·L^-1,最大反应速率Vm为16.5 g·（L·h）^-1。采用等电点提高碳源纯度,提取液中总碳水化合物和还原糖浓度最终可分别达到61.5±2.6 g·L-1和21.1±0.9 g·L-1,碳氮比（C/N）从17.9增加至24.3,有潜力用于污水处理厂反硝化或者其它生物转化过程。更多还原

【Abstract】 Food waste contains a large amount of organic carbon sources and has the potential for resource recycling.In this paper,the organic carbon sources of food waste were separated by enzymatic method and isoelectric point precipitation method.The results showed that the synergistic effect of amylase and glucosidase could effectively isolate the carbon sources in the food waste.On the basis of experiment,box-Behnken response surface method was used to optimize the key parameters of enzymatic hydrolysis.Under the optimal conditions of temperature 57.6 ℃,pH value 6.25,substrate concentration 20%（calculated as TS）,enzyme content 0.8%,the ratio of amylase and glucosidase is 3:1,the extraction rate of carbon source is 76.9%.The complex enzymatic hydrolysis process was in line with michaelis-Menten kinetic model.The constant Km is 31.7 g·L^-1,and the Vm is 16.5 g ·（L·h）^-1.The isoelectric point was used to improve the purity of carbon source,and the total carbohydrate and reducing sugar concentration in the extract could finally reach 61.5±2.6 g·L^-1 and 21.1±0.9 g·L^-1,respectively.The carbon-nitrogen ratio（C/N） increased from 17.9 to 24.3,which has the potential to be used in denitrification of sewage treatment plants or other biological transformation processes.更多还原