Quantitative proteomic analysis of the sugarcane defense responses incited by Acidovorax avenae subsp. avenae causing red stripe

【作者】 周敬如； Hui-Dong Sun； Ahmad Ali； Talha Javed； Philippe C.Rott； Hua-Ying Fu； 高三基；

【Author】 Jing-Ru Zhou;Hui-Dong Sun;Ahmad Ali;Talha Javed;Philippe C.Rott;Hua-Ying Fu;San-Ji Gao;National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University;CIRAD, UMR BGPI;BGPI, Univ Montpellier, CIRAD, INRA, L’institut Agro;

【机构】 National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University； CIRAD, UMR BGPI； BGPI, Univ Montpellier, CIRAD, INRA, L’institut Agro；

【摘要】 The causal agent of red stripe, Acidovorax avenae subsp. avenae(Aaa), can incite significant economic losses during sugarcane production. Proteomics is a useful tool for the comprehensive identification of defense response proteins in plants. In this study, we used analysis of iTRAQ-based proteomics to identify proteins that were produced in sugarcane in defense to Aaa in two Saccharum spp. cultivars: one was resistant(ROC22) and the other one(MT11-610) was susceptible to red stripe. Defense response was evaluated at 0 h(mock-inoculation) and 24 h post-inoculation with the bacterial pathogen, and 10,502 high-confidence proteins and 2,889 differentially expressed proteins(DEPs) were identified. These DEPs included 1,027 proteins(671 upregulated, 354 downregulated) in ROC22 and 1,130 proteins(566 upregulated, 564 downregulated) in MT11-610. Gene ontology analysis revealed that most DEPs were clustered in the metabolic, single-organism metabolic, and oxidation-reduction processes, and in catalytic activity. In a KEGG analysis, these proteins belonged to common pathway such as metabolic pathways, secondary metabolite biosynthesis, and biosynthesis of phenylpropanoids. Thirty-six DEPs were identified, especially epoxide hydrolase 1, dynamin-related protein 1 C-like, cytochrome oxidase subunit I, FK506-binding protein, acetoacetyl CoA thiolase isoform 2, anthocyanidin 3-o-glucosyltransferase, ubiquitin carboxyl-terminal hydrolase 4, protein disulfide isomerase-like protein, and jumonji-like transcription factor family protein. These proteins were characterized by enhanced expression with ≥ 1.5-fold increases in resistant ROC22 versus susceptible MT11-610, and are therefore biomarker candidates in the sugarcane response against Aaa. These data could be very useful in molecular breeding for sugarcane resistance to red stripe.更多还原

【Abstract】 The causal agent of red stripe, Acidovorax avenae subsp. avenae(Aaa), can incite significant economic losses during sugarcane production. Proteomics is a useful tool for the comprehensive identification of defense response proteins in plants. In this study, we used analysis of iTRAQ-based proteomics to identify proteins that were produced in sugarcane in defense to Aaa in two Saccharum spp. cultivars: one was resistant(ROC22) and the other one(MT11-610) was susceptible to red stripe. Defense response was evaluated at 0 h(mock-inoculation) and 24 h post-inoculation with the bacterial pathogen, and 10,502 high-confidence proteins and 2,889 differentially expressed proteins(DEPs) were identified. These DEPs included 1,027 proteins(671 upregulated, 354 downregulated) in ROC22 and 1,130 proteins(566 upregulated, 564 downregulated) in MT11-610. Gene ontology analysis revealed that most DEPs were clustered in the metabolic, single-organism metabolic, and oxidation-reduction processes, and in catalytic activity. In a KEGG analysis, these proteins belonged to common pathway such as metabolic pathways, secondary metabolite biosynthesis, and biosynthesis of phenylpropanoids. Thirty-six DEPs were identified, especially epoxide hydrolase 1, dynamin-related protein 1 C-like, cytochrome oxidase subunit I, FK506-binding protein, acetoacetyl CoA thiolase isoform 2, anthocyanidin 3-o-glucosyltransferase, ubiquitin carboxyl-terminal hydrolase 4, protein disulfide isomerase-like protein, and jumonji-like transcription factor family protein. These proteins were characterized by enhanced expression with ≥ 1.5-fold increases in resistant ROC22 versus susceptible MT11-610, and are therefore biomarker candidates in the sugarcane response against Aaa. These data could be very useful in molecular breeding for sugarcane resistance to red stripe.更多还原