【作者】 郭晓强； 何浩岚； 谢磊； 赵海龙； 莫雨晓； 谢延峥； 潘翊； 何金洋；

【Author】 GUO Xiao-qiang;He Hao-lan;Xie lei;ZHAO Hai-long;MO Yu-xiao;XIE Yang-zheng;PAN Yi;HE Jin-yang;Institute of Tropical Medicine, Guangzhou University of Chinese Medicine;Guangzhou Eighth People’s Hospital;Guangzhou Haizhu District Center for Disease Control and Prevention;

【机构】 广州中医药大学热带医学研究所； 广州市第八人民医院； 广州市海珠区疾病预防控制中心；

【摘要】 目的构建可进行SIV复制的裸小鼠模型,为抗艾滋病病毒药物筛选的体内实验提供一种廉价、易于操作的艾滋病小动物模型。方法向BALB/c裸小鼠腹腔内接种已感染猴免疫缺陷病毒(SIVmac251)的TZM-b1细胞,构建SIV体内复制的裸小鼠动物模型,通过采集小鼠血浆,进行逆转录实时荧光定量PCR法(RT-PCR)检测血浆中SIVmac251病毒载量,并且通过HE染色法检测小鼠主要器官的组织学改变,通过高效联合抗逆转录病毒疗法(HAART)对该模型进行药物干预,验证该模型的抗病毒药物评价效果。结果 1、BALB/c裸小鼠成功接种已感染猴免疫缺陷病毒(SIVmac251)的TZM-b1细胞。2、连续七周在BALB/c裸小鼠血浆里检测出SIVmac251病毒载量。3、在小鼠主要器官出现了组织学改变。4、验证了HAART对小鼠体内SIVmac251复制的抑制作用。结论构建了支持SIV复制的小动物模型,该模型能够支持SIVmac251病毒的复制,并表现出组织学病理变化,为研究抗艾滋病药物体内抑制病毒复制提供了廉价、易于操作的小动物模型。更多还原

【Abstract】 Objectives:Establishment of a SIV infected animal model for providing an inexpensive, easy-to-operate small animal model for in vivo experiments in anti-HIV drug screening.Methods:BALB/c nude mice were intraperitoneally inoculated with TZM-b1 cells infected with SIVmac251. Plasma viral load were tested by reverse transcription real-time fluorescent quantitative PCR(RT-PCR).Pathologicall changes of major organs in mice were detected by HE staining.Mice were subjected to drug intervention withHighly Active Anti-Retroviral Therapy(HAART) to evaluate the efficacy of the SIV-infected mouse model.Results:1, BALB/c nude mice were successfully inoculated with TZM-b1 cells infected with SIVmac251. 2, SIV load could be detected in the plasma of the BALB/c nude mice model for seven consecutive weeks. 3, There were marked histological changes in the main organs of the mice model. 4, The inhibition effect of HAART on SIVmac251 virus replication in mice was verified. Conclusion:A small animal model of SIV infection was established. This model can support the in vivo replication of the SIVmac251 and showed pathological changes in main organs.This mouse model provides a cheap, easy-to-operate small animal model for studying anti-HIV drugeffect in vivo.更多还原