【作者】 王宇； 游颖； 田圆； 孙海月； 李霞； 王秀娟； 王玉华； 刘景圣；

【Author】 Wang Yu;You Ying;Tian Yuan;Sun Haiyue;Li Xia;Wang Xiujuan;Wang Yuhua;Liu Jingsheng;College of Food Science and Engineering,Jilin Agricultural University;Jilin province Innovation Center for Food Biological Manufacture;National Processing Laboratory for Soybean Industry and technology;National Engineering Laboratory for Wheat and Corn Deep Processing;

【机构】 吉林农业大学食品科学与工程学院； 吉林省食品生物制造创新中心； 国家大豆产业技术体系加工实验室； 小麦和玉米深加工国家工程实验室；

【摘要】 肠道菌群与饮食引起的脂代谢紊乱密切相关,而影响高脂血症的微生物对健康有着重要的影响。从东北酸菜中分离得到的戊糖片球菌PP04具有良好的胃肠道耐受性并且能在肠道中稳定定植,在本文中,我们研究了其抗高脂血症作用。以高脂饲料补充戊糖片球PP04干预喂养C57BL/6N小鼠,确保每日摄入量为10~9 CFU/小鼠,观察其抗高脂血症的效果。8周后,检测与高脂血症、肝损伤及炎症相关的指标。结果表明,戊糖片球菌PP04可以有效地抑制高脂饮食诱导小鼠体重和体脂的增加,下调血清总胆固醇、总甘油三脂、低密度脂蛋白、游离脂肪酸、瘦素、谷丙转氨酶、谷草转氨酶及肿瘤坏死因子α的水平。戊糖片球菌PP04的这些功能与AMPK通路介导的通过上调PPARγ促进肝脏脂肪分解和下调SREBP1c,FAS,SCD1抑制肝脏脂肪生成密切相关。此外,戊糖片球菌PP04通过激活Nrf2/CYP2E1通路有效地改善高脂饮食诱导的氧化应激,提高了包括超氧化物歧化酶和谷胱甘肽过氧化物酶在内的抗氧化剂活性。与对照组相比,高脂饮食导致厚壁菌门和拟杆菌的比值显著升高,而戊糖片球菌PP04干预后,该比值略有降低。戊糖片球菌PP04通过调节盲肠中与脂质代谢和炎症相关Mogibacterodies、Mucispirillum、Bacterodies和Coprococcus的相对丰度,改善了高脂饮食诱导的小鼠肠道菌群失调。本研究证实了戊糖片球菌PP04具有独特的治疗价值,通过激活AMPK信号通路和调节肠道菌群改善了高脂饮食引起的高脂血症。更多还原

【Abstract】 The gut microbiota has been linked to diet-induced lipid metabolism disorder,and microorganisms that influence hyperlipidemia have important health implications.The Pediococcus pentococcus PP04 isolated from the Northeast pickled cabbage had well gastrointestinal tolerance and could stably colonize in the intestine.This study investigated the anti-hyperlipidemia effects of P.pentococcus PP04.C57 BL/6 N mice with a high-fat diet were treated with P.pentosaceus PP04 at a daily consumption of 10~9 CFU/mouse to evaluate the anti-hyperlipidemia effect.After eight weeks,the indicators of hyperlipidemia,liver injury and inflammation were measured.The results suggested that P.pentosaceus PP04 supplementation could effectively inhibit weight gain and body fat induced by high-fat diet and reduced the levels of TC,TG,LDL,FFA,leptin,ALT,AST,LPS and TNF-α in serum.This indicated that P.pentosaceus PP04 not only inhibited lipid accumulation in the liver but also improved liver injury and inflammatory response caused by high-fat diet. These functions of P.pentosaceus PP04 were associated with AMPK-mediated stimulation of hepatic lipolysis through upregulation of PPARy and suppression of hepatic lipogenesis,partly via downregulation of SREBP1 c,FAS,and SCD1.Furthermore,the P.pentosaceus PP04 effectively improved high-fat diet induced oxidative stress by activating the Nrf2/CYP2 E1 pathway which enhanced antioxidant activity including SOD and GSH-Px.In addition,the high-fat diet resulted in a significant increase in the ratio of Firmicutes/Bacteroidetes compared with control group,however,the intervention of P.pentosaceus PP04 slightly reduced this value.The supplement of P.pentosaceus PP04 ameliorated gut microbiological dysbiosis in high-fat diet fed mice by regulating the relative abundance of Mogibacteriaceae,Mucispirillum,Bacterodies and Coprococcus in cecum which were associated with lipid metabolism and inflammation.This study confirms the unique therapeutic value of P.pentosaceus PP04 in preventing of highfat diet induced hyperlipidemia which activating AMPK signaling and regulating the gut microbiota.更多还原