【作者】 高凝轩； 孟宪军； 郎宇曦； 李斌；

【Author】 Gao Ningxuan;Meng Xianjun;Lang Yuxi;Li Bin;College of Food Science,Shenyang Agricultural University;

【机构】 沈阳农业大学食品学院；

【摘要】 首先通过对国内种植的7个品种的黑果腺肋花楸多酚组分及细胞抗氧化活性进行测定。采用多元相关性分析,考察不同多酚组分对细胞抗氧化活性的贡献。针对上述试验结果,为提高抗氧化活性,以黑果腺肋花楸果渣为原料,利用热-酸-醇处理将其中高聚原花青素转化为花青素。在单因素试验中,分别考察乙醇浓度、液料比、盐酸浓度、Fe³⁺浓度、处理温度、处理时间6个因素对花青素得率的影响。在此基础上,采用全因子试验设计优化工艺参数。利用Q-TOF-MS和UPLC分析产物中花青素组分。结果表明,高聚原花青素是黑果腺肋花楸多酚的主要物质,而花青素与细胞抗氧化活性的相关性更高。将黑果腺肋花楸果渣按50:1的液料比与乙醇浓度95%、盐酸浓度4%、Fe³⁺浓度0.2 mg/mL的溶液混合,于80℃下处理30 min,其中高聚原花青素可有效转化为花青素,花青素得率为11.59 mg/g。转化所得花青素为矢车菊素,占到总花青素含量的51.3%。转化处理后,细胞抗氧化活性提高4.77倍。本研究可为黑果腺肋花楸花青素的工业化生产提供理论依据。更多还原

【Abstract】 Firstly,the polyphenol components and cellular antioxidant activities of seven varieties from chokeberry produced in China were determined.Multiple correlation analysis was used to investigate the contribution of different polyphenol components to cellular antioxidant activities.Based on the above experimental results,in order to improve the antioxidant activity,the polymeric proanthocyanidins from chokeberry pomace were converted into anthocyanidins by hot-acid-alcohol treatment.In single factor experiment,the effects on the yield of anthocyanidins was investigated from six aspects:ethanol concentration,liquid-solid ratio,hydrochloric acid concentration,Fe³⁺ion concentration,treatment temperature,treatment time.On this basis,optimal conversion conditions were determined by full factorial design.The composition of anthocyanidin was analyzed by Q-TOF-MS and UPLC.The results showed that proanthocyanidins were the main components of polyphenols in chokeberry,and the correlation between anthocyanidins and cellular antioxidant activities was higher.The yield of anthocyanidins was 11.59 mg/g when the pomace was treated with 95% ethanol concentration,50:1 liquid-solid ratio,4% hydrochloric acid and 0.2 mg/mL Fe³⁺ ion concentration at 80℃ for 30 min.The newly generated anthocyanidins were identified as cyanidin,which accounting for 51.3% of the total anthocyanidins.The cellular antioxidant activity increased 4.77 folds by conversion treatment.These research results can provide a theoretical basis for the industrial production of anthocyanidins from chokeberry.更多还原