【作者】 陈晴； 柳雅馨； 张子怡； 李可； 刘斌； 岳田利；

【Author】 Chen Qing;Liu Yaxin;Zhang Ziyi;Li Ke;Liu Bin;Yue Tianli;College of Food Science and Engineering,Northwest A&F University;Laboratory of Quality and Safety Risk Assessment for Agro-products,Ministry of Agriculture;National Engineering Research Center of Agriculture Integration Test;Zhejiang Academy of Science & Technology for Inspection & Quarantine;

【机构】 西北农林科技大学食品科学与工程学院； 农业部农产品质量安全风险评价实验室； 中国农业综合试验国家工程研究中心； 浙江检验检疫科学技术研究院；

【摘要】 单月桂酸甘油酯(GML)是一种脂肪酸单甘油酯,具有广谱抗菌作用,然而对革兰氏阴性菌尤其是沙门氏菌无效。为了提高GML对沙门氏菌的杀菌率,将该试剂分别与在溶液中呈酸性、中性和碱性的消毒剂乳酸(LA)、氯化十六烷基吡啶(CPC)和磷酸三钠(TSP)3种消毒剂组合。结果表明,1%GML和复合消毒剂0.5%GML-0.025%LA对沙门氏菌有较强的杀菌作用。涡旋振荡处理后杀菌率分别达到99.92%和98.29%,说明涡旋振荡处理可以增强GML对沙门氏菌外膜的破坏。在模拟鸡肉浸泡、漂洗过程的试验中,0.5%GML-0.025%LA复合物在短时间(0 h)内杀菌效果较好,更适合瞬时杀菌;而在较长时间(4 h)内,1%GML杀菌效果较好,说明它更适合长期抑菌。用Ci7600比色仪、TA.XT Plus质谱仪和高光谱成像光谱仪测定了鸡肉样品的颜色和质地特征。与对照组相比,所有样品经复合消毒剂处理后没有显著差异。综上所述,GML是一种潜在的、良好的鸡肉加工消毒剂。更多还原

【Abstract】 Glycerol monolaurate(GML) is a monoglycerol ester of the fatty lauric acids,which has a wide-spectrum antimicrobial capacity,but fails to gram-negative bacteria,especial Salmonella.To enhance the bactericidal rate of GML for Salmonella,this reagent was combined with three disinfectants:lactic acid(LA),cetylpyridinium chloride(CPC),and trisodium phosphate(TSP),which can present acid,neutral,and alkaline in solution.The results showed that the 1% GML and a complex disinfectant(0.5% GML-0.025% LA) could powerfully sterilize Salmonella.Their bactericidal rates respectively were able to achieve 99.92% and 98.29% with the vortex treatment,indicating that the vortex treatment could improve GML to destruct the outer membrane of Salmonella.During the simulation test of the soaking and rinse processing of chicken,for a short time(0 h),the effect of 0.5% GML-0.025% LA compound was better and more suitable for instantaneous sterilization than others,while for a long time(4 h),1 % GML exhibited a better bactericidal effect,which indicated it to be more suitable for long-term bacteriostasis.The characterization of color and texture for chicken samples were determined using Colormeter Ci7600 TA.XT Plus and Hyper-spectral Imager,which demonstrated that all samples treated by these complex disinfectants were not significantly different.In conclusion,GML was a potential and superior disinfectant for the chicken process.更多还原