【作者】 胡景华； 张丽珍； 廖春华； 江武军； 曾志将； 颜伟玉；

【Author】 HU Jing-Hua;ZHANG Li-zheng;LIAO Chun-Hua;JIANG Wu-Jun;ZENG Zhi-Jiang;YAN Wei-Yu;Honeybee Research Institute,Jiangxi Agricultural University;Apiculture Research Institute of Jiangxi Province;

【机构】 江西农业大学蜜蜂研究所； 江西省养蜂研究所；

【摘要】 [目的]基于免移虫育王技术探究幼虫信息素酯类的三种组合3E(1.0%甲基亚油酸酯、16.0%甲基亚麻酸酯、35.0%甲基油酸酯)、4E(4.5%甲基棕榈酸酯、1.0%甲基亚油酸酯、16.0%甲基亚麻酸酯、35.0%甲基油酸酯)和10E(4.5%甲基棕榈酸酯、2.5%甲基硬脂酸、35.0%甲基油酸酯、1.0%甲基亚油酸酯、16.0%甲基亚麻酸酯、3.5%乙基棕榈酸酯、1.5%乙基硬脂酸酯、18.0%乙基油酸酯、0.5%乙基亚油酸酯、17.5%乙基亚麻酸酯)在育王中对中华蜜蜂Apis cerana cerana蜂王质量的影响。[方法]利用中华蜜蜂免移虫育王生产器培育蜂王,在幼虫60-64h时向王台内注入1μL配制好的酯类混和物(浓度梯度分别为0%、0.1%、1.0%、10.0%),待蜂王出房后测定蜂王初生重、胸重、胸宽以及单侧卵巢管数量,并利用荧光定量PCR测定蜂王卵巢卵黄蛋白原基因Vitellogenin(Vg),昆虫储存蛋白基因hexamerin 70b (hex70b)和hexam erin110(hex110)的表达量。[结果]免移虫育王过程中添加3E,不同浓度处理蜂王的初生重均显著增加,但蜂王胸宽和胸重均无显著变化;0.1%和1.0%浓度处理蜂王卵巢管数显著增加;不同浓度处理Fg和hex70b的表达量均无显著变化,但1.0%和10.0%浓度处理蜂王卵巢的hex110表达量显著升高。4E试验组,只有10.0%浓度处理蜂王的初生重和卵巢管数显著上升,其它浓度处理对蜂王的个体指标均无显著影响;0.1%和1.0%浓度处理Vg的表达量显著上升,10.0%处理下hex70b的表达量显著增加,1.0%和10.0%浓度处理hex110表达量也显著上升。10E试验组,各个浓度处理蜂王的初生重、Vg和hex110的表达量均显著下降,但蜂王的卵巢管数和胸部指标无显著变化,10.0%的浓度处理hex70b的表达量也显著降低。[结论]育王过程中添加幼虫信息素酯类对中华蜜蜂育王质量存在影响,1.0%的3E显著增加蜂王的初生重、卵巢管数和hex110的表达量;10.0%的4E也显著增加蜂王的初生重和卵巢管数,同时蜂王卵巢hex70b和hex110的表达水平显著上升。更多还原

【Abstract】 [Aim] The objective of this study is to investigate the effects of 3 E(1.0%methyl linoleate,16.0%methyl linolenate,35.0%methyl oleate),4 E(4.5%methyl palmitate,1.0%methyl linoleate,16.0%methyl linolenate,35.0%methyl oleate) and 10 E(4.5% methyl palmitate,2.5% methyl stearate,35.0% methyl oleate,1.0% methyl linoleate,16.0% methyl linolenate,3.5% ethyl palmitate,1.5% ethyl stearate,18.0% ethyl oleate,0.5% ethyl linoleate,17.5% ethyl linolenate) on the quality of queen reared by queen-rearing technology without larvae-grafting.[Methods] Queen-rearing technology without larvae-grafting was employed to rear queen of Apis cerana cerana.1μL of combination of brood pheromone esters with concentration gradient of 0,0.1%,1.0% and 10.0% was added into the queen cells respectively using a micro sampling syringe injector when queen larvae was 60-63 h old.The weight,number of ovariole tube in one side,the weight and width of thorax of newly-emerged queens were tested and compared.And gene expression of Vitellogenin(Vg),hexamerin70 b(hex70 b) and hexamerin110(hex110)was quantified by qRT-PCR.[Results]The results showed that the newly-emerged weight was increased significantly(P<0.05) in all concentration treatments of 3 E,but no significant effects were found in the thorax index and ovarioles number of reared queens compared with control(P> 0.05).The queens have higher expression of hex110 gene in 1.0% and 10.0% concentration of(P<0.05),while in Vg and hex70 b genes there was no significant difference among the experimental groups of 3 E.The weight and ovarioles number was significantly increased when the queen was reared with10.0% of 4 E(P<0.05).Expression of Vg in queen’s ovary was significantly higher in 0.1%and 1.0% treatment of 4 E(P<0.05),while higher expression of hex70 b existed in 10.0%(P<0.05),and hex110 in 1.0% and 10.0% treatment of 4 E(P <0.05).In 10 E,the newly-emerged weight,expression of Vg and hex110 gene were all lower in all treatments than those in control,while in the ovarioles number and thorax index no significant difference were found.Expression of hex70 b reduced significantly only in 10% treatment of 10 E(P<0.05).[Conclusion]Combinations of brood pheromone esters applied in queen-rearing process could affect the queen’s quality,1% treatment of 3 E significantly increased the newly-emerged weight and the ovarioles number,and increased the expression of hex110 gene significantly in queen ovary.In 10% treatment of 4 E,the newly-emerged weight,the ovarioles number and the expression of hex70 b and hex110 of queen were all increased significantly.更多还原