Identification of differentially expressed microRNAs associate with glucose metabolism in different organs of blunt snout bream(Megalobrama amblycephala)

【作者】 缪凌鸿； Yan Lin； Wen-Jing Pan； Xin Huang； 戈贤平； Ming-Chun Ren； Qun-Lan Zhou； Bo Liu；

【Author】 Ling-Hong Miao;Yan Lin;Wen-Jing Pan;Xin Huang;Xian-Ping Ge;Ming-Chun Ren;Qun-Lan Zhou;Bo Liu;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;

【机构】 Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences；

【摘要】 Blunt snout bream(Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequently-used fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of high-starch diet(HSD) induced lipid metabolic disorder by identifying microRNAs(miRNAs) controlled pathways in glucose and lipid metabolism in fish using high-throughput sequencing technologies. Small RNA libraries derived from intestine, liver, and brain of HSD and normalstarch diet(NSD) treated M. amblycephala were sequenced and 79, 124 and 77 differentially expressed miRNAs(DEMs) in intestine, liver, and brain of HSD treated fish were identified, respectively. Bioinformatics analyses showed that these DEMs targeted hundreds of predicted genes were enriched into metabolic pathways and biosynthetic processes, including peroxisome proliferator-activated receptor(PPAR), glycolysis/gluconeogenesis, and insulin signaling pathway. These analyses confirmed that miRNAs play crucial roles in glucose and lipid metabolism related to high wheat starch treatment. These results provide information on further investigation of DEMs related mechanism dysregulated by high carbohydrate diet.更多还原

【Abstract】 Blunt snout bream(Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequently-used fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of high-starch diet(HSD) induced lipid metabolic disorder by identifying microRNAs(miRNAs) controlled pathways in glucose and lipid metabolism in fish using high-throughput sequencing technologies. Small RNA libraries derived from intestine, liver, and brain of HSD and normalstarch diet(NSD) treated M. amblycephala were sequenced and 79, 124 and 77 differentially expressed miRNAs(DEMs) in intestine, liver, and brain of HSD treated fish were identified, respectively. Bioinformatics analyses showed that these DEMs targeted hundreds of predicted genes were enriched into metabolic pathways and biosynthetic processes, including peroxisome proliferator-activated receptor(PPAR), glycolysis/gluconeogenesis, and insulin signaling pathway. These analyses confirmed that miRNAs play crucial roles in glucose and lipid metabolism related to high wheat starch treatment. These results provide information on further investigation of DEMs related mechanism dysregulated by high carbohydrate diet.更多还原