【作者】 王伟伟； 李庆刚； 张莉鸽； 崔洁； 于浩； 王晓玉； 唐鸿志； 许平；

【Author】 Wang Weiwei;Li Qinggang;Zhang Lige;Cui Jie;Yu Hao;Wang Xiaoyu;Tang Hongzhi;Xu Ping;Shanghai Jiao Tong University;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences;

【机构】 上海交通大学； 中国科学院天津工业生物技术研究所；

【摘要】 本多环芳烃(PAHs)和二恶英类化合物(包括硫,氮和氧杂环)具有毒性,诱变性和致癌性,人们对其引发的环境污染和健康问题的日益关注。能够利用芳香族化合物进行生长的各类微生物对于此类污染场地的生物修复过程,以及有机和无机状态之间的地球碳循环过渡中发挥着至关重要的作用。微生物修复是一种可用于治理多环芳烃和二恶英污染环境的潜在技术。然而,多种污染物的生物修复过程通常会面临过多种芳烃类、二恶英类和有机溶剂的共存的现状,对于修复复合污染环境,相关的微生物学和遗传学基础还有待进一步研究。从联苯污染土壤中分离的恶臭假单胞菌株B6-2可以代谢降解多种芳香族化合物,包括美国环境保护局规定的13种优先控制的PAHs污染物。菌株B6-2是一种生物安全菌株,可用于环境生物技术和环境修复。在本项研究中,借助基因组学和蛋白质组学方法来鉴定联苯(BP)及联苯、二苯并呋喃(DBF)、苯并噻吩(DBT)和咔唑(CA)的混合物代谢过程中的关键基因。对菌株B6-2包含一个6.37Mb的全基因组完整序列,其遗传分析揭示了多种芳香化合物中心代谢系统。使用联苯作为唯一碳源,通过蛋白质组学分析鉴定了总共1,241种蛋白质,其中254种蛋白质具有明显差异表达(114种上调和140种下调)。此外,基于转录组数据对菌株B6-2在BP培养下转录水平的动态变化进行分析,发现多条与代谢芳香化合物相应的基因簇有明显的转录水平上调。我们对菌株B6-2广谱的芳香化合物代谢能力及多组学分析芳香化合物代谢网络的研究表明,菌株B6-2是在复合污染的生物修复中具有潜在应用价值,同时是研究恶臭假单胞菌的生物化学、遗传学和进化方面的潜在研究模型。更多还原

【Abstract】 Polycyclic aromatic hydrocarbons(PAHs) and dioxin-like compounds, including sulfur, nitrogen and oxygen heterocycles, are widespread and toxic environmental pollutants. A wide variety of microorganisms capable of growing with aromatic polycyclic compounds are essential for processes of bioremediation of contaminated sites and for transitioning the Earth?s carbon cycle between organic and inorganic states. Here, cells of Pseudomonas putida B6-2 grown in the presence of biphenyl can simultaneously degrade PAHs and their derivatives, even when they were present as mixtures, and tolerate high concentrations of extremely toxic solvents.. We used functional genomics and proteomics methods to identify candidate genes associated with the catabolism of biphenyl and a mixture of biphenyl(BP), dibenzofuran(DBF), benzothiophene(DBT), and carbazole(CA). Using biphenyl as the sole carbon source, total of 1,241 proteins were identified by proteomic analysis, of which 254 proteins had altered expression(114 up-regulated and 140 down-regulated). Genetic analysis of the 6.37 Mb genome of strain B6-2 reveals central metabolic systems of aromatic compounds and genetic mechanism for the solvent tolerance. We observed dynamic changes in transcriptional levels with BP, including in metabolic pathways of aromatic compounds, chemotaxis, effluc pumps, and transporters potentially involved in adaptation to the PAHs. Our studies on the highly versatile activities of strain B6-2 reveal it to be a potentially useful model for bioremediation and for investigating the biochemical, genetic, and evolutionary aspects of P. putida.更多还原