【作者】 邹旋； 张晓敏； 张仁利； FENG Tiejian； SUN Ying； Meng Jun； HUANG Yalan； XIONG Linghong； PENG Bo； FANG Shisong；

【Author】 ZOU Xuan;ZHANG Xiaomin;FENG Tiejian;SUN Ying;Meng Jun;HUANG Yalan;XIONG Linghong;PENG Bo;FANG Shisong;ZHANG Renli;Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention;

【机构】 深圳市疾病预防控制中心病原生物研究所； Institute of Pathogen Biology, Shenzhen Center for Disease Control and Prevention；

【摘要】 目的探讨酶联免疫吸附法(ELISA)检测血清中严重急性呼吸综合征冠状病毒2(SARS-CoV-2)特异性抗体--免疫球蛋白(Ig)A、IgG在新型冠状病毒肺炎(COVID-19)诊断中的临床应用价值,以及探索IgA是否可以作为比IgG更灵敏的COVID-19诊断血清标志物。方法选取47例COVID-19确诊患者为研究对象,收集其年龄、性别、发病时间、入院时间、出院时间及采样时间等信息,采集血清样本124份,利用ELISA试剂盒和酶标仪检测患者血清中SARS-CoV-2特异性IgA和Ig G抗体含量。结果患者中21-40岁组人数最多,占比40.43%;41-60岁组占比36.17%;61岁以上患者只有10.64%。IgG检测阳性率较高,灵敏度最好,以Ig G1(59.26%)和IgG3(44.44%)亚型为主;IgA的敏感性次于IgG,几乎所有IgA阳性标本都伴随Ig G阳性。IgG和IgA阳性组患者的年龄高于抗体阴性组,15岁以下儿童和70岁以上老年患者均为Ig A阴性。抗体阳性组与阴性组患者的住院天数及病程时长之间的差异无统计学意义。IgG抗体水平随着发病时间延长而持续升高;而IgA抗体则在患者发病后的前三周逐渐升高,然后随着发病时间延长而逐渐下降。结论 ELISA法检测COVID-19患者血清中SARS-CoV-2特异性IgG抗体的敏感性较高,有良好的辅助诊断价值。更多还原

【Abstract】 Objective To explore the clinical value of serological test for SARS-CoV-2 specific antibody-IgA and IgG in the serum of COVID-19 patients by using ELISA. To confirm whether IgA can be a better serological diagnosis marker of COVID-19 than IgG. Methods A total of 124 serum samples from 47 COVID-19 patients were detected for SARSCoV-2 specific IgA and IgG by using ELISA kits and microplate reader. Two to 4 samples per patient were collected at different time points. The age, gender, onset time, admission time, discharge time and sampling time of patients were collected for data analysis.Results The patients aged 21-40 accounted for 40.43% followed by the patients aged 41-60(36.17%). The patients over 61 were the least, only 10.64%. SARS-CoV-2 IgG showed better sensitivity for the diagnosis of COVID-19 than SARS-CoV-2 IgA. The major IgG subtypes included IgG1(59.26%) and IgG3(44.44%). Of note, almost all the IgA positive samples were also positive for IgG. The ages of IgA and IgG positivepatients were significantly higher than those of antibody negative ones. Most patients were between 21 to 60 years old in the antibody positive group. IgA was negative in the samples of both children under 15 and old patients above 70 years old. There was no significant difference between the positive group and the negative group in the length of hospitalization and duration. The level of IgG antibody kept increasing with the time of onset, while IgA level displayed a rising trend in the first three weeks after onset then decreased gradually in the following two weeks.Conclusion SARS-CoV-2 specific IgG test by using ELISA has a very good auxiliary diagnostic value for COVID-19.更多还原