【作者】 王佳慧； 李禤； 贾丹； 张有鹏； 卢俊仕； 白小明； 刘军龙； 刘爱红； 王锦明； 关贵全； 王雅； 罗建勋； 殷宏； 李有全；

【Author】 WANG Jia-Hui;LI Xuan;JIA Dan;ZHANG You-peng;LU Jun-shi;BAI Xiao-ming;LIU Jun-long;LIU Ai-hong;WANG Jin-ming;GUAN Gui-quan;Wang Ya;LUO Jian-xun;YIN Hong;LI You-quan;State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;Lanzhou university of Technology;

【机构】 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室； 兰州理工大学；

【摘要】 益生菌是指一种可以改善动物肠道菌群平衡,有利于动物生长发育的一种有活性的微生态制剂。该研究从健康新鲜牛粪便中分离菌种,利用形态学和16S rRNA基因扩增测序,并对其中益生菌进行抑菌性试验,从中筛选候选益生菌株。结果表明,所有分离的菌株包括球菌和杆菌,除柠檬酸杆菌、大肠杆菌、产气克雷白(氏)杆菌为革兰氏阴性菌,其余均为革兰氏阳性菌;这些菌株依次为1株假肠膜明串株菌,3株粪肠球菌,2株海氏肠球菌,3株屎肠球菌,1株绿浅气球菌,3株魏斯氏菌,1株类鸟肠球菌,2株产气克雷白(氏)杆菌,1株柠檬酸杆菌,2株枯草芽孢杆菌,3株大肠杆菌,2株甲基营养型芽孢杆菌,2株蜡样芽胞杆菌。其中屎肠球菌、粪肠球菌、枯草芽孢杆菌、海氏肠球菌、假肠膜明串株菌都有一定的耐药性。本研究从健康牛的新鲜粪便中分离和筛选益生菌,为后续牛源益生菌的进一步研究提供了候选菌株。更多还原

【Abstract】 Probiotics is an active microecological preparation that can improve the balance of the intestinal flora of animals and facilitate the growth and development of animals. In this study, the bacteria were isolated and identified from the feces of healthy cattle, based on morphology and sequencing of 16 S rRNA gene, and the antibacterial activity of some probiotics were carried out. The results of PCR and sequential analysis showed that some bacteria were isolated and identified, and they included 3 strains of Enterococcus faecalis, 2 strains of Enterococcus faecium, 3 strains of Enterococcus faecium, 1 strain of Phytophthora sp., 3 strains of Weiss spp., 1 strain of Enterococcus, 2 strains Klebsiella pneumoniae, 1 strain of Citrobacter sp., 2 strains of Bacillus subtilis, 3 strains of Escherichia coli, 2 strains of Methylotrophic Bacillus, 2 strains of Bacillus cereus. Among them, Enterococcus faecium, Enterococcus faecalis, Bacillus subtilis, Enterococcus faecalis, and pseudo-intestinal strains have certain drug resistance. The candidate probiotics strains lay a foundation of further research on animal microecological preparation.更多还原