三种不同来源犬MSCs及其外泌体对软骨细胞增殖的影响

【作者】 陈胜锋； 张贝莹； 王丙云； 罗冬章； 詹小舒； 陈志胜； 计慧琴； 白银山； 刘璨颖； 李东升；

【机构】 佛山科学技术学院生命科学与工程学院； 广东维赛生物科技有限公司；

【摘要】 目的:研究骨髓、脂肪和脐带(UC-MSC)来源的MSCs及其外泌体对犬的软骨细胞增殖能力的影响,阐明MSC修复关节损伤的机制。方法:本实验通过设立空白对照组、完全培养基对照组、生理盐水对照组、MSCs组及外泌体(exosomes,EXO)组,采用transwell共培养法研究骨髓、脂肪和脐带来源MSCs对关节软骨细胞(articular chondrocyte,AC)的影响。结果:三种不同来源的MSCs均能显著增强AC的细胞活性(P<0.01),其中脐带源MSC(UC-MSCs)的作用最强;通过研究UC-MSCs来源的EXO和不舍外泌体的条件培养基(conditioned medium,CM)对AC增殖的影响,结果发现UC-MSCs不含外泌体的CM和UC-MSC-EXO均能促进AC增殖(P<0.01),但不含外泌体的CM效果更好。以上结果提示,不同来源的UC-MSCs均可通过促进软骨细胞的增殖而修复关节损伤,其机制是主要通过分泌外泌体和其它成分来促进软骨细胞增殖。更多还原

【Abstract】 In this study a blank control group,a complete medium control group,and a saline control group were set up and the method of transwell co-culture was used to explore the effect of bone marrow,fat and umbilical cord derived MSCs on the proliferation of articular chondrocyte(AC).The results showed that these three sources of MSCs could significantly enhance the cellular activity of AC(P<0.01),of which UC-MSC had the best enhancement effect.The effects of UC-MSC-derived exosomes(EXO) and their conditioned medium(CM) with no EXO on AC were further compared.It was found that the CM with no EXO of UC-MSCs promoted the proliferation of AC more significantly than the UC-MSCs exosomes(P<0.01).更多还原