【作者】 张健； 刘咏； 王军辉；

【Author】 Zhang Jian;Liu Yong;Wang Junhui;School of Food Science and Engineering,Hefei University of Technology;

【机构】 合肥工业大学食品科学与工程学院；

【摘要】 黄山石耳多糖主要是1,6-Glcp链接的刚性葡聚糖,是一种具有免疫活性和凝胶性质的天然高分子。本文利用氢氧化钠、尿素和水溶剂体系简便快速的溶解石耳多糖,用环氧氯丙烷作为交联剂成功制备新型石耳多糖水凝胶。研究主要利用流变仪,对水凝胶交联过程中交联剂浓度、多糖含量、凝胶化时间以及凝胶化温度等影响因素进行重点探究,考察溶胶-凝胶转变过程中的流变学行为。通过扫描电子显微镜(SEM)、傅立叶变换红外光谱(FT-IR)以及X射线衍射(XRD)对黄山石耳多糖水凝胶的表面形貌和组织结构进行分析,并研究了交联剂浓度、多糖浓度对水凝胶溶胀性能的影响。水凝胶的细胞毒性通过RAW264.7进行研究。研究主要结果如下:(1)石耳多糖、环氧氯丙烷体系的凝胶化受到温度和时间影响,凝胶行为是时间和温度的函数关系。多糖含量的增加和交联剂浓度的升高都可以使凝胶点下降;(2)根据红外光谱结果可知,石耳多糖与环氧氯丙烷都存在于凝胶体系中,整个网络结构也已经成功构建。(3)当多糖含量为10%、交联剂浓度为12%,60℃下处理20 h制备得到力学性能和溶胀性能良好的石耳多糖水凝胶;(4)石耳多糖水凝胶对RAW264.7巨噬细胞具有良好的细胞相容性。本论文利用氢氧化钠/尿素体系成功创建出石耳多糖水凝胶,并阐明水凝胶的交联过程,结构与性能之间的关系。这些基础研究结果不仅克服传统水凝胶力学性能和智能水凝胶活性上的不足,为黄山石耳材料的开发提供新途径,也对环境友好的生物质水凝胶的设计、制备及应用提供重要科学依据和理论。更多还原

【Abstract】 Umbilicaria esculenta polysaccharides,mainly contains rigid 1,6-Glcp linked dextran,has immune activity and gel property.The NaOH/urea aqueous solution dissolved polysaccharides,and a polysaccharides-based hydrogel was successfully prepared.The technologies(SEM,FT-IR and XRD) analysed the surface morphology and structure of hydrogels,in addition,the swelling properties and cytotoxicity were explored.The main results were as following:(1) Sol-gel transition of the Umbilicaria esculenta polysaccharides and epichlorohydrin system is affected by temperature and time,and the gel behavior is a function of time and temperature.(2) FTIR showed that the gelation process was the typical of epichlorohydrin crosslinking.(3) hydrogels had good mechanical properties and swelling properties:10% polysaccharide,12% epichlorohydrin,60℃ heated for 30 min.(4) The hydrogel displayed good cytocompatibility to RAW264.7 cells.These basic researchs provide valuable information for construction,and development of biobased hydrogels with different properties from renewable resource,and useful to application of Umbilicaria esculenta polysaccharides in food industry.更多还原