【作者】 贾飞； 戴瑞彤； 李兴民； Jozef Kokini；

【Author】 Jia Fei;Dai Ruitong;Li Xingmin;Jozef Kokini;College of Food Science and Nutritional Engineering,China Agricultural University;Department of Food Science,Purdue University;

【机构】 中国农业大学食品科学与营养工程学院； 普渡大学食品科学系；

【摘要】 本试验利用生物可降解的玉米蛋白膜制备了用于表面增强拉曼检测的拉曼基底。通过在基底表面修饰"倒金字塔"结构和引入纳米金材料来提高信号响应,并用作微型检测平台实现对水中绿脓毒素的快速灵敏检测。基底中间的"倒金字塔"结构大小为2微米为底面边长和2.1微米高,间距是2微米。拉曼基底的大小,形态以及分布用扫描电镜进行表征。通过对比试验,可以发现纳米金材料的引入可以显著增强其拉曼信号响应。在最优条件下,用所制备的拉曼基底对绿脓毒素进行检测。不需要任何的预处理,整个检测可以在30分钟之内完成。跟常见的市售拉曼硅制基底或玻璃基底相比,本文所用的玉米蛋白膜属于生物可降解材料,在环保和可持续发展方面凸显其优势。并且该基底可以在1 h之内完全溶于乙醇溶液,金材料也能进一步回收重复利用。在未来,这种基于玉米蛋白膜制备的生物可降解拉曼基底一定能广泛应用在食源性致病菌和其他化学物质检测当中。更多还原

【Abstract】 In this paper,a biodegradable zein Surface Enhanced Raman Spectroscopy(SERS) substrate,functionalized with gold nanoparticles(AuNPs) and in an inverted pyramid structure was developed,and worked as a lab-on-a-chip platform to enable the sensitive and rapid detection of pyocyanin in drinking water.The inverted pyramid structure in the middle of the chip,with a base of 2 μm by 2 μm with a periodicity of 2 μm(from center to center),and hight of 2.1 μm,has been proved to be able to improve the Raman single.The size,distribution and morphology of the zein substrate were characterized by scanning electron microscopy(SEM).The enhancement factor of the substrate can be further increased by the introduction of AuNPs,and a comparable experiment were conducted to evaluated the Raman single enhancement of the AuNPs.Under the optimized experiment condition,the SERS substrate can be successfully used in the pyocyanin detection.No complex pre-treatment of sample needed,the developed SERS detection method can complete the measurement within 30 mins.Most importantly,the zein-based substrate shows great advantages in the biodegradability compared with the commercial available silicon or glass substrate.The zein substrate can be easily re-dissolved in the ethanol solution within 1 hour and the gold material can be harvested.In the foreseeable future,the zein-based biodegradable biosensor can be used in food-borne pathogens and chemicals detection.更多还原