【作者】 王晓瞳； 许加超； 王联珠； 付晓婷； 高昕； 赵艳芳；

【Author】 Wang Xiaotong;Xu Jiachao;Wang Lianzhu;Fu Xiaoting;Gao Xin;Zhao Yanfang;College of Food Science and Engineering,Ocean University of China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;

【机构】 中国海洋大学食品科学与工程学院； 中国水产科学院黄海水产研究所；

【摘要】 建立了一种高效的从海带中提取一碘酪氨酸(MIT)和二碘酪氨酸(DIT)的方法。首先使用微波对海带粉进行处理,然后利用超声水浴辅助胰酶水解提取海带中的碘氨基酸。首先进行单因素实验,根据实验结果利用响应面实验进一步优化提取条件,最终得到最佳提取条件为微波功率250 W,微波时间4.7 min,0.31 g胰酶,水解4.22 h。使用高效液相色谱光电二极管阵列(HPLC-PDA)建立了一种快速简单,低成本的检测MIT和DIT的方法。检出限范围为50.62至320.58 ng/g。利用扫描电子显微镜(SEM)用于观察不同提取方法对海带细胞的损伤程度。采用尺寸排阻色谱与电感耦合等离子体质谱联用(SEC-ICP-MS)研究了原始海带中的有机碘和酶水解后的海带的分子量分布。更多还原

【Abstract】 A rapid and efficient method for extracting monoiodotyrosine(MIT) and diiodotyrosine(DIT) from kelp was successfully developed.The kelp samples were first pretreated with microwaves,before the MIT and DIT were extracted with an ultrasonic water bath.A response surface experiment was used to optimize the extraction conditions,and ultimately the best conditions for extraction were successfully identified,which were a microwave power of 250 W,a microwave time of 4.7 min,an addition of 0.31 g of pancreatin,and 4.22 h in th ultrasonic water bathe.A simple and low-cost high-performance liquid chromatography photodiode array(HPLC-PDA) detection method for MIT and DIT was established.The detection limits ranged from 50.62 to 320.58 ng/g.Scanning electron microscopy(SEM) was used to observe the degree of damage to the kelp cells from the different extraction methods.The molecular weight distribution of the organic iodine of the original kelp and the kelp after enzymatic hydrolysis was studied by size-exclusion chromatography inductively coupled plasma mass spectrometry(SEC-ICP-MS).更多还原