【作者】 伯继芳； 杨灵； 徐怀德；

【Author】 Bo Jifang;Yang Ling;Xu Huaide;College of Food Science and Engineering,Northwest A&F University;

【机构】 西北农林科技大学食品科学与工程学院；

【摘要】 在本研究中,从杏鲍菇（Pleurotus eryngii,PE）中提取的多糖经过化学修饰得到三种硫酸化衍生物(SPEP-60_1:2、SPEP-60_1:5、SPEP-60_1:8),探讨硫酸化修饰后多糖的结构与生物活性之间的关系。通过化学方法,气相色谱,液相色谱,傅里叶变换红外光谱（FT-IR）,紫外光谱和抗氧化活性（羟基自由基清除力、DPPH·自由基清除力、超氧自由基清除力）以及对餐后消化关键酶的抑制活性（α-淀粉酶、α-葡萄糖苷酶）等对硫酸化杏鲍菇多糖衍生物的理化性质和生物活性进行了比较研究。结果表明,杏鲍菇多糖的改性成功,硫酸化后多糖的理化性质发生了变化,生物活性均有所改变。所有的结构修饰多糖的生物活性与剂量均呈正相关,且除DPPH·自由基清除力没有增加外,其他的活性均显著增强,特别是对酶活性的抑制作用。在这些硫酸化多糖中,具有相对较高的多糖和糖醛酸含量,较低分子量（Mw）的SPEP-60_1:8对DPPH·自由基清除力最强但超氧阴离子自由基清除力和α-葡萄糖苷酶的抑制活性最弱;然而具有相对最高取代度（DS）和甘露糖含量的SPEP-60_1:5在对DPPH·自由基清除力最弱。结构修饰后这些结果表明,硫酸化多糖体外生物活性是DS,Mw,单糖组成和糖醛酸含量等的综合效应的结果。更多还原

【Abstract】 In the present study,the polysaccharide extracted from Pleurotus eryngii was chemically modified to obtain three sulfated derivatives(SPEP-60_1:2、SPEP-60_1:5、 SPEP-60_1:8),and investigate the relationship between the structure and biological activity of polysaccharides after sulfation modification.By chemical methods,gas chromatography,liquid chromatography,Fourier transform infrared spectroscopy（FT-IR）,ultraviolet spectroscopy and antioxidant activity（hydroxyl radical scavenging power,DPPH · free radical scavenging power,superoxide radical scavenging power） and the inhibitory activities of key digestion enzymes（α-amylase,α-glucosidase） after lunch,the physicochemical properties and biological activities of sulfated Pleurotus eryngii polysaccharide derivatives were compared.The results showed that the modification of Pleurotus eryngii polysaccharide was successful,and the physicochemical properties of polysaccharides changed after sulfation,and the biological activities were changed.The biological activity of all the structurally modified polysaccharides was positively correlated with the dose,and except for the DPPH · free radical scavenging power,the other activities were significantly enhanced,especially the inhibition of enzyme activity.Among these sulfated polysaccharides,relatively high polysaccharide and uronic acid content,lower molecular weight（Mw） SPEP-60_1:8 has the strongest DPPH · radical scavenging power but superoxide anion radical scavenging power and the inhibitory activity of the a-glucosidase was the weakest;however,the SPEP-60_1:5with the highest degree of substitution（DS） and mannose content had the weakest scavenging power to DPPH · free radicals.After structural modification,these results indicate that the in vitro biological activity of the sulfated polysaccharide may be the result of a combined effect of DS,Mw,monosaccharide composition,and uronic acid content.更多还原