【作者】 朱新军； 仓世龙； 焦德杰； 张静霞； 张连营；

【Author】 Zhu Xinjun;Cang Shilong;Jiao Dejie;Zhang Jingxia;Zhang Lianying;College of Life Science,Dezhou University;College of Environmental Science and Engineering;

【机构】 德州学院生命科学学院； 南开大学环境科学与工程学院；

【摘要】 本研究采用共沉淀法制备了磁性Fe₃O₄纳米粒子,在其表面进行Au³⁺还原,获得Fe₃O₄/Au纳米粒子,并利用2-巯基-5-苯并咪唑磺酸钠（2-Mercapto-5-benzimidazolesulfonic acid,MBISA）对Fe₃O₄/Au进行修饰,制备得到Fe₃O₄/Au-MBISA复合纳米颗粒.应用光子相关光谱（PCS）和振动样品磁强计（VSM）等对Fe₃O₄/Au-MBISA复合纳米颗粒进行表征,结果显示该复合纳米颗粒的平均水合粒径为74 nm,具有超顺磁性,饱和磁化强度（Ms）为44 emu/g.MBISA修饰使复合颗粒的等电点（pI）由pH=5.7变为3.6,在较大pH值范围内其表面都保持较高的负电性.研究了Fe₃O₄/Au-MBISA对溶菌酶的吸附及解吸特性,包括离子强度、吸附时间、温度、pH等因素的影响.结果显示Fe₃O₄/Au-MBISA复合纳米颗粒对溶菌酶的最大吸附量为346μg/mg.在最佳释放条件下,Fe₃O₄/Au-MBISA复合纳米颗粒的溶菌酶释放率可达100%.Fe₃O₄/Au-MBISA复合纳米颗粒具有良好的重复利用性能,经过7次吸附-洗脱循环后,其溶菌酶吸附量仍可保持在91%以上.将Fe₃O₄/Au-MBISA复合纳米颗粒应用于蛋清溶菌酶的提取纯化,结果显示,所提取到的溶菌酶具有较高的纯度,SDS-PAGE呈单一条带.综上所述,Fe₃O₄/Au-MBISA复合纳米颗粒是一种溶菌酶的良好载体,可应用于蛋清中溶菌酶的提取纯化研究及生产中。更多还原

【Abstract】 2-Mercapto-5-benzimidazolesulfonic acid（MBISA） modified Fe₃O₄/Au nanoparticles（Fe₃O₄/Au-MBISA nanoparticles） were synthesized in aqueous solution and characterized by photo correlation spectroscopy（PCS） and vibrating sample magnetometer（VSM）.The average diameter of Fe₃O₄/Au-MBISA nanoparticles was 74 nm with a polydisperion（PDI） of 0.244,and the nanoparticles behaved superparamagnetically with saturated magnetization（Ms） of 44 emu/g.The isoelectric point（pI） of the Fe₃O₄/Au-MBISA nanoparticles was located at pH 3.6 and a highly negative surface charge in a wider range of pH was observed.The so-obtained Fe₃O₄/Au-MBISA nanoparticles were capable of specific adsorbing lysozyme.The maximum amount of lysozyme adsorbed on 1.0 mg Fe₃O₄/Au-MBISA nanoparticles was 346 μg.The lysozyme desorption behavior was studied and the lysozyme recovery from Fe₃O₄/Au-MBISA nanoparticles approached 100% under optimal conditions,and the reusability studies showed that the nanoparticles could maintain about 91% of the initial lysozyme adsorption capacity after 7 repeated adsorption-elution cycles.The Fe₃O₄/Au-MBISA nanoparticles were used in the purification of lysozyme from chicken egg white,which was verified by a single SDS-PAGE band.Therefore,the obtained Fe₃O₄/Au-MBISA nanoparticles exhibited excellent performance in the direct purification of lysozyme from egg white.更多还原