【作者】 刘楠； 曾帅； 孙永； 王珊珊； 赵峰； 周德庆；

【Author】 Liu Nan;Zeng Shuai;Sun Yong;Wang Shanshan;Zhao Feng;Zhou Deqing;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology;

【机构】 中国水产科学研究院黄海水产研究所海洋国家实验室海洋药物与生物制品功能实验室；

【摘要】 通过Box Behnke试验设计和响应面法优化羊栖菜多酚纳米微胶囊的制备条件,获得粒径为178nm,包封率为42.3%,Zeta电位为+37mV的微胶囊。制备得到的羊栖菜多酚纳米微胶囊在PBS7.4的缓冲液中可缓慢释放48小时。通过UV辐射剂量、羊栖菜多酚纳米微胶囊的浓度及小鼠成纤维细胞L929细胞与微胶囊共培养的时间研究羊栖菜多酚纳米微胶囊对UV照射的L929细胞的抗光损伤的影响。结果表明UV辐射剂量越大,L929细胞受到损伤越严重,UV辐射半致死剂量为0.15J/cm~2。羊栖菜多酚纳米微胶囊对L929细胞的保护作用随浓度的增大呈现先增强后减弱的趋势,在为90μg/mL时对L929细胞的保护作用最强。将L929细胞与羊栖菜多酚纳米微胶囊共培养4h后进行UV照射,保护作用最明显,但随着时间的延长,保护作用开始下降。羊栖菜多酚纳米微胶囊对UV照射后的L929细胞具有修复作用,浓度为80μg/mL时修复作用最强。此时,L929细胞内超氧化物歧化酶和谷胱甘肽过氧化物酶的活性较空白对照显著增强,丙二醛的含量显著下降。以上实验表明羊栖菜多酚纳米微胶对UV照射L929细胞具有保护及修复作用。更多还原

【Abstract】 Hijichi japonicus polyphenol nanocapsules preparation conditions had been optimized through the Box-Behnken experimental design.The nanocapsules with particle size of 178 nm,loading efficiency of 42.3% and Zeta potential of +37 mV had been obtained.The Hijichi japonicus polyphenol nanocapsules can be released slowly for 48 hours in PBS 7.4.UV irradiation dose,concentration of polyphenol nanocapsules and time of co-culture of L929 cells and nanocapsules were used to study the effect of polyphenol nanocapsules on UV irradiated L929 cells.The results showed that the higher the dose of UV irradiation,the more serious the damage to L929 cells,and the semi-lethal dose of UV irradiation was 0.15 J/cm~2.The protective effect of polyphenol nanocapsules on L929 cells was first enhanced and then weakened with the increase of concentration,and the protective effect was strongest in the case of 90 pa/mL.UV irradiation was performed after 4 h of co-culture of L929 cells and polyphenol nanocapsules for the most obvious protective effect.However,with the extension of time,the protective effect began to decline.The polyphenol nanocapsules had the most effective repair effect on L929 cells after UV irradiation,when the concentration was 80 pa/mL.At this point,the activity of superoxide dismutase and glutathione peroxidase in L929 cells was significantly enhanced and the content of malondialdehyde was significantly decreased.The above experiments showed that the Hijichi japonicus polyphenol nanocapsules had protective and servicing effects on UV irradiated L929 cells.更多还原