【作者】 李娜； 周德庆； 吕世伟； 刘楠； 王珊珊； 马玉洁；

【Author】 Li Na;Zhou Deqing;Lü Shiwei;Liu Nan;Wang Shanshan;Ma Yujie;The Laboratory of Food Engineering and Nutrition,Yellow Sea Fisheries Research Institute;College of Food Science and Technology,Shanghai Ocean University;

【机构】 中国水产科学研究院黄海水产研究所海洋国家实验室海洋药物与生物制品功能实验室； 上海海洋大学食品学院；

【摘要】 目的:对鳕鱼鱼鳔来源酶解肽的抗氧化活性与延缓H₂O₂诱导2BS细胞衰老作用的研究。方法:利用复合蛋白酶对鳕鱼鱼鳔进行酶解,将得到的酶解产物进行G-15葡聚糖凝胶柱层析。对分离纯化组分进行傅里叶红外光谱扫描、紫外光谱扫描、氨基酸组成分析与分子量测定。检测鱼鳔肽对DPPH·,HO·与O2·-自由基体外清除能力与Fe²⁺螯合能力。通过H₂O₂诱导2BS细胞建立早期细胞衰老模型,对鱼鳔肽对氧化诱导衰老细胞的细胞增殖率、ROS活性氧含量、β-半乳糖苷酶含量与细胞凋亡率进行检测。结果:通过分离纯化得到两个组分,分别命名为SWP-Ⅰ与SWP-Ⅱ。体外清除自由基能力实验结果显示两个组分对DPPH·,HO·与O2·-自由基具有一定的清除能力,且具有较好的Fe²⁺螯合能力。对H₂O₂诱导的早期衰老2BS细胞,鳕鱼鱼鳔肽能够有效提高细胞增殖率,降低ROS活性氧含量、β-半乳糖苷酶含量与细胞凋亡率。结论:通过酶解得到的鳕鱼鱼鳔肽具有较好的抗氧化能力并对H₂O₂诱导的早期衰老2BS细胞具有一定的保护作用,能够有效地延缓细胞衰老。更多还原

【Abstract】 Objective:Antioxidant activity of enzymatic peptides from Swimming bladder of codfish and its anti-aging effects on 2 BS induced by H₂O₂.Methods:Swimming bladder peptides were obtained by enzymatic hydrolysis with compound protease and then isolated by chromatographic method with G-15.The fractions were characterized by FT-IR,UV scanning spectrum amino acid composition analysis and molecular weight determination.The antioxidant such as scavenging activities on DPPH ·,HO · and O² ·- and chelating activity of Fe²⁺ of peptides were evaluated in vitro.The cell viability,intracellular reactive oxygen species production,content of β-galactosidase and apoptosis rate in human fetal lung diploid fibroblast cells induced by H₂O₂ were detected.Results:Two bioactive peptides（SWP-Ⅰ and SWP-Ⅱ） were isolated by chromatographic method with G-15.The results of free radical scavenging showed that SWP-Ⅰ and SWP-Ⅱ possessed good scavenging activities on DPPH ·,HO · and O² ·- and good Fe²⁺ chelating activity.Both of two peptides reduced intracellular reactive oxygen species production,content of β-galactosidase and apoptosis rate in human fetal lung diploid fibroblast cells induced by H₂O₂.Conclusion:The enzymatic peptides from swimming bladder of codfish possessed antioxidant activities and could protect the early aging cells induced by H₂O₂ effectively.更多还原