【作者】 邹娅雪； 付晓婷； 许加超； 高昕；

【Author】 Zou Yaxue;Fu Xiaoting;Xu Jiachao;Gao Xin;College of Food Science and Engineering,Ocean University of China;

【机构】 中国海洋大学食品与科学学院；

【摘要】 本文研究了琼胶寡糖(Agaro-oligosaccharides,AOS)对2,2-偶氮二(2-甲基丙基咪)二盐酸盐(AAPH)诱导损伤的斑马鱼的抗氧化作用及机制。采用高效液相色谱法对酸法制备的龙须菜琼胶寡糖组分进行分析。优化建立了AAPH诱导的斑马鱼模型,并以斑马鱼胚胎存活率、活性氧生成率和细胞死亡率等指标评价了不同浓度的琼胶寡糖的抗氧化活性。实验结果表明琼胶寡糖(25,50,100μg/mL)对AAPH(15 mmol/L)引起的氧化应激损伤的斑马鱼有保护作用,且呈剂量依赖性。其中最优质量浓度100μg/mL AOS能显著提高斑马鱼胚胎存活率(96.88%,P<0.05),并能显著降低AAPH引起的斑马鱼体内活性氧生成率(100.53%,P<0.05)和细胞死亡率(101.69%,P<0.05)。本研究首次利用斑马鱼模型研究了AOS的抗氧化活性,揭示了其抗氧化机制主要是清除体内大量活性氧生成和阻止细胞死亡损伤。与已报到的海藻糖类物质相比,AOS具有更好的抗氧化活性,为其作为抗氧化剂的应用提供了理论基础。更多还原

【Abstract】 Antioxidant activities and mechanisms of agaro-oligosaccharides(AOS) were studied in 2,2-azobis(2-methylpropylimidazole) dihydrochloride(AAPH)-induced zebrafish model.The composition of acidic hydrolyzed AOS was determined by HPLC.The optimal AAPH induced zebrafish oxidative stress model was established,and the antioxidant activities of AOS with different concentrations were investigated by determination of embryo survival rate,reactive oxygen production rate,and cell death rate.The results showed that AOS(25,50,100 μg/mL) had a protective effect on oxidative stress induced by AAPH(15 mmol/L) in a dose-dependent manner.The optimal concentration of 100 μg/mL AOS could significantly increase the survival rate of zebrafish embryos(96.88%,P <0.05),and could significantly reduce the reactive oxygen species production rate(100.53%,P<0.05) and cell death rate(101.69%,P<0.05) of AAPH-induced zebrafish.This is the first report to study the antioxidant activities of AOS using zebrafish model.The antioxidant mechanism of AOS was investigated to be associated with the elimination of large amounts of reactive oxygen species and the prevention of cell death in vivo.AOS exhibited better antioxidant activities as compared with other reported carbohydrates from seaweed,which indicated its potential to be used as an excellent antioxidant.更多还原