【作者】 马晴晴； 李雪； 殷焕顺； 艾仕云；

【Author】 Qingqing Ma;Xue Li;Huanshun Yin;Shiyun Ai;College of Chemistry and Material Science, Shandong Agricultural University;

【机构】 山东农业大学化学与材料科学学院；

【摘要】 光电化学生物分析是基于光电化学过程和化学/生物识别过程建立起来的一种新的分析方法。本文研究了一种利用类石墨相碳化氮（g-C₃N₄）作为光电转换材料,Au-ZIF-8作为一种MOF材料增强g-C₃N₄的光电化学信号,TiO₂作为磷酸基团连结分子,以及抗坏血酸作为电子供体的灵敏的、可用于蛋白激酶活性分析和抑制剂筛选的光电化学分析方法。实验中特定的底物肽在蛋白激酶A（PKA）的催化作用下,发生磷酸化,引入的磷酸基团与TiO₂结合,成功的构建了生物传感器。该生物传感器对蛋白激酶A活性的检测呈现高灵敏度,检出限为0.02 U/mL,线性方程为I（nA）=259.298+136.89logc。通过抑制剂鞣花酸对PKA活性抑制作用的研究,进一步扩宽了制备的传感器的应用性。因此,本文所提出的光电化学方法在激酶活性和激酶抑制剂的筛选的检测方面具有巨大的潜力。更多还原

【Abstract】 Photoelectrochemical（PEC） biological analysis is a new method of analysis based on photoelectrochemical process and chemical/biological identification process.A highly sensitive and selective PEC biosensor is fabricated for the detection of activity of protein kinase A（PKA） and screen out inhibitors, which is based on the specific connection of visible-light active graphite-like carbon nitride（g-C₃N₄） and phosphorylated peptides by TiO₂, and ascorbic acid provide electrons during detection process.After the immobilized peptides are phosphorylated under PKA catalysis, they can be specifically identified and binded with TiO₂.With the good photoactivity of g-C₃N₄ and electrons ascorbic acid provided, the fabricated biosensor presents high sensitivity, low detection limit（0.02 U/mL, S/N=3） for PKA detection,whose linear equation is I（nA）=259.298+136.89 logc.The applicability of this PEC biosensor was further testified by the evaluation of PKA inhibition by ellagic acid.Therefore, the developed PEC method has great potential in screening of kinase inhibitors and highly sensitive and selective detection of kinase activity.更多还原