【作者】 王冬梅； 钱媛媛； 李永新；

【Author】 Dongmei Wang;Yuanyuan Qian;Yongxin Li;College of Chemistry and Materials Science, Anhui Normal University;

【机构】 安徽师范大学化学与材料科学学院；

【摘要】 基于金纳米线电极良好的空间分辨率,构建了一种检测三磷酸腺苷ATP的电化学DNA传感器。采用激光拉制再刻蚀的方法制备并表征了半径<30 nm,长度约100nm的纳米线电极,并将其应用于可再生的电化学传感器的构建。透射电子显微镜用于金纳米线电极的表面形貌表征。实验中,标记了亚甲基蓝(MB)的DNA双链通过分子自组装固定在金纳米线电极表面。当加入ATP后,MB标记的适配子DNA由于与目标分子的特异性结合,从双链DNA中解脱下来,根据解脱前后亚甲基的电信号的不同对加入的ATP进行定量的测定。我们将传统的金盘电极与此纳米线电极制备的传感器做了比较,结果表明此纳米线电极DNA传感器具有较高的信噪比,选择性好,能很好的应用于大鼠脑脊髓液中ATP的检测。这种传感机制可拓展成为一种通用的检测平台,用于多种生物分子的超灵敏检测。更多还原

【Abstract】 Take advantage of the excellent spatiotemporal resolution of small diameter electrode, a duplex aptamer-based sensor has been developed by use of single Au nanowire electrode for sensitive and selective determination of ATP.In this paper, a single Au nanowire electrode(30 nm radius and 100 length approximately) was fabricated and characterized, and then applied to the construction of reproducible electrochemical aptasensor based on the formation of Au-S bond between duplex DNA tagged with MB and Au surface.In the presence of ATP, the MB-labeled aptamer dissociated from the duplex DNA due to the strong specific affinity between aptamer and the targeted DNA which lead to the reduction of MB signals.In addition, the sensor performance on conventional electrode was also investigated for comparison.The results have showed the proposed E-AB sensors on single nanoelectrode represents an increasing signal-to-noise, reusable platform for highly sensitive detection of ATP or other target analytes.更多还原