【作者】 刘云清； 李小雨； 杨瑞英； 张小华； 陈金华；

【Author】 Yunqing Liu;Xiaoyu Li;Ruiying Yang;Xiaohua Zhang;Jinhua Chen;State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University;

【机构】 湖南大学化学化工学院化学生物传感与计量学国家重点实验室；

【摘要】 末端转移酶(TdT)是一种识别单链DNA3′-OH末端的免模板DNA聚合酶。血晶素/G四联体是一种可同时作为NADH氧化酶及HRP仿酶的DNA酶。通过TdT引入大量血晶素/G四联体,我们发展了一种基于DNA的电化学生物传感器,成功应用于碱性磷酸酶活性检测。更多还原

【Abstract】 Terminal deoxynucletidyl transferase(TdT) is a template independent DNA polymerase and could catalyze the sequential addition of dNTPs at the 3′-OH group of an oligonucleotide primer to produce non-specific sequence.Hemin/G-quadruplex DNAzyme has been reported to be simultaneously served as an NADH oxidase and HRP-mimicking DNAzyme.Taking TdT to introduce lots of hemin/G-quadruplex DNAzymes, we developed a sensitive and selective DNA-based electrochemical biosensor for AP activity assay.更多还原