【作者】 邹桂征； 谭肖； 张斌；

【Author】 Guizheng Zou;XiaoTan;Bin Zhang;School of Chemistry and Chemical Engineering, University of Shandong;

【机构】 山东大学化学与化工学院；

【摘要】 光谱型和波长可选择的电化学发光（ECL）对构建多色ECL分析策略至关重要。我们用自制的ECL光谱采集系统,通过光谱成像记录标记物CdSe纳米颗粒的ECL辐射,采用夹心免疫法将Cd Se标记到CA125作为目标分子,构建一种光谱型免疫分析策略。CdSe纳米颗粒ECL发射峰在555nm,半峰宽39nm,可以有效地检测CA125,线性范围10^-4U/mL-1 U/mL,检测下限为5×10^-5U/mL。ECL相比于荧光分析信噪比高,这也使得光谱型ECL免疫分析可以成为一种有前途的基于纳米颗粒的多色分析策略。更多还原

【Abstract】 Spectrum-based and color-selective electrochemiluminescence（ECL） is crucial for multiple-colored ECL assay strategy.Herein, a spectrum-based ECL immunoassay of high color-purity was performed on a home-made ECL acquiring system by spectrally imaging the ECL emission of highly-passivated CdSe nanocrystals（NCs）, i.e.the ECL tags, which were labeled onto sandwich-typed immuno-complexes with CA125 as target molecule.The ECL tags could give off efficient ECL around 555 nm with the full width at half-maximum of 39 nm, so that the spectrum-based ECL strategy could color-selectively determine CA125 with linear response range from 10^-4 U/mL to 1 U/mL and the limit of detection of 5×10^-5 U/mL.The intrinsically improved signal/noise ratio of ECL to fluorescence might eventually enable the spectrum-based ECL immunoassay a promising alternative to the NCs based multiple-colored fluorescent assay strategy.更多还原