【作者】 赵亚囡； 霍丹群； 侯长军；

【Author】 Yanan Zhao;Danqun Huo;Changjun Hou;Key Laboratory of Biorheology Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University;

【机构】 重庆大学生物工程学院生物流变科学与技术教育部重点实验室；

【摘要】 抗坏血酸（AA）,多巴胺（DA）以及尿酸（UA）是人体内非常重要的三种生物小分子,它们在生物体内含量的异常与许多疾病相关[1]。AA、DA以及UA都具有很好的电化学活性,因此电化学方法检测这三种物质受到了科学家的广泛关注[2]。因此,在本工作中,我们使用辛胺辅助水热法合成了具有层状二硫化钼材料,并通过煅烧的方法得到碳包覆的二硫化钼纳米管（MoS₂@C）。在优化的实验条件下,由MoS₂@C修饰在玻碳电极（GCE）表面,实现了AA、DA以及UA氧化峰的有效分离（图1）。所制备的MoS₂@C/GCE传感器不仅可以分别检测AA、DA以及UA并可以用于同时检测三种物质的混合液,表现了优异的催化性能,AA的检测线性范围为0.8μM到1.3mM, DA的检测线性范围为0.3μM到70μM, UA的检测范围为1μM到1mM。此外,该材料修饰的电极还显示了良好的选择性,对于钠盐、葡萄糖和氨基酸类化合物,响应量很小。并且进一步在实际血清样的中进行检测也有可观的结果。因此,在生物分析检测方面具有很好的应用前景。更多还原

【Abstract】 Ascorbic acid（AA）, dopamine（DA） and uric acid（UA） are three kinds of small biological molecules, which is very important in the human body.These three spices are all electroactive molecules and have been widely studied by electroanalytical techniques.Therefore, in this work, carbon-anchored ultrathin MoS₂ nanosheets, which is prepared via a facile hydrothermal method and Subsequently calcination.We employed carbon-anchored ultrathin MoS₂ nanosheets（MoS₂@C） as efficient modifying materials on electrode to simultaneous detection of DA, AA and UA.Under the optimized operational conditions, the oxidation potentials of these three species have a clear separation.The prepared MoS₂@C/GCE sensor can not only detect AA, DA and UA,but also can be used for simultaneous detection in mixed liquid, which showed excellent catalytic activity.The biosensor has good linear relation to the AA, DA and UA concentrations in a range 0.8μM to 1.3 mM, 0.3μM to 70μM and 1μM to 1 mM respectively.The modified electrode also shows good selectivity.As a novel nanomaterial, it shows potential application in biochemical analysis.更多还原