【作者】 徐沛瑶； 王士斌； 陈爱政；

【Author】 Xu Pei-yao;Wang Shi-bin;Chen Ai-zheng;College of Chemical Engineering, Huaqiao University;Fujian Provincial Key Laboratory of Biochemical Technology (Huaqiao University);

【机构】 华侨大学化工学院； 福建省生物化工技术重点实验室(华侨大学)；

【摘要】 吲哚菁绿是一种具有广泛的生物成像应用前景的近红外荧光染料,但由于其在水溶液中的不稳定性、自聚集性、易与蛋白质的非特异性结合及非靶向性等缺点,使得游离吲噪菁绿无法达到理想的光热效果。本文通过超临界流体抗溶剂法构建吲哚菁绿纳米颗粒,并通过静电吸附作用在其表面包覆上聚赖氨酸。吲哚菁绿-聚赖氨酸纳米颗粒有效延长了吲哚菁绿的近红外光热治疗效果,并提高了吲哚菁绿对细胞成像的敏感性和精准性。结果表明,吲噪菁绿-聚赖氨酸纳米颗粒在肿瘤诊断和光热治疗中有较好的应用前景。更多还原

【Abstract】 Indocyanine green(ICG)has garnered enormous attention from researchers as a near-infrared(NIR)fluorescence dye for a wide-variety of biomedical applications.Nevertheless,the applicability of free ICG in achieving the desired therapeutic efficacy is often limited due to several drawbacks such as instability and extensive aggregation in aqueous solutions,and rapid elimination from the body,and poor targeting ability,among.others.Briefly,ICG NPs that produced by supercritical antisolvent(SAS)process were encapsulated in poly-L-lysine(PLL)through electrostatic adsorption to obtain ICG-PLL NPs.ICG-PLL NPs have shown prolonged tumor ablation than that of free ICG due to NIR laser-induced hyperthermia.More importantly,the ICG-PLL NPs significantly promoted more accurate and sensitive imaging of ICG in’cells.The results demonstrated that ICG-PLL NPs based on supercritical fluid technology offer enormous potential in tumor diagnosis and photothermal tumor therapy.更多还原