Complete mitochondrial genome of the meadow moth,Loxostege sticticalis(Lepidoptera:Pyraloidea:Crambidae),compared to other Pyraloidea moths

【作者】 Hong-Fang Ma； Xi-Xi Zheng； Ming-Hui Peng； Hai-Xu Bian； Miao-Miao Chen； 刘彦群； 江幸福； Li Qin；

【Author】 Hong-Fang Ma;Xi-Xi Zheng;Ming-Hui Peng;Hai-Xu Bian;Miao-Miao Chen;Yan-Qun Liu;Xing-Fu Jiang;Li Qin;Insect Resource Center for Engineering and Technology of Liaoning Province,College of Bioscience and Biotechnology,Shenyang Agricultural University;State Key laboratory for Biology of Plant Diseases and Insect Pest,Institute of Plant Protection,Chinese Academy of Agricultural Sciences;

【机构】 Insect Resource Center for Engineering and Technology of Liaoning Province,College of Bioscience and Biotechnology,Shenyang Agricultural University； State Key laboratory for Biology of Plant Diseases and Insect Pest,Institute of Plant Protection,Chinese Academy of Agricultural Sciences；

【摘要】 We present the complete mitogenome of the meadow moth,Loxostege sticticalis L.,a serious economic pest of both crops and weeds worldwide.This circular genome is 15,218 bp in length,with a typical gene orientation and order comparable to other sequenced Pyraloidea insects.The genome composition of the major strand exhibits highly AT bias(80.82%),with a slightly positive AT skew indicating the occurrence of more As than Ts.The L.sticticalis mitogenome has a total of 130 bp of intergenic spacer sequences spread over 15 regions,ranging in size from 1 to 48 bp,of which only two are common among the 23 total Pyraloidea moths that have data collected on the mitogenome(one is located between tRNAGln and ND2 with variation change in length and a limited sequence conservation,and the other is located between tRNASer(UCN) and ND1 with a conserved 6 bp motif ’ATACTA’).The AT-rich region of 331 bp in the genome is comprised of non-repetitive sequences but contains an ATAGN motif followed by a poly-T stretch of 17 bp,a microsatellite-like(TA)11 element preceded by an ATTTA motif,and a poly-A stretch upstream tRNAMet.These conserved structures identified in the AT-rich region are presented in all of the sequenced Pyraloidea species.We provide a mitogenome-based phylogeny of Pyraloidea species,in which L.sticticalis shares close ancestry to Ostrinia species with substantial evidence.Our phylogenetic analyses strongly divide Crambidae into two sister lineages.The mitogenome dataset also supports the basal split between Pyraustinae and Spilomelinae.更多还原

【Abstract】 We present the complete mitogenome of the meadow moth,Loxostege sticticalis L.,a serious economic pest of both crops and weeds worldwide.This circular genome is 15,218 bp in length,with a typical gene orientation and order comparable to other sequenced Pyraloidea insects.The genome composition of the major strand exhibits highly AT bias(80.82%),with a slightly positive AT skew indicating the occurrence of more As than Ts.The L.sticticalis mitogenome has a total of 130 bp of intergenic spacer sequences spread over 15 regions,ranging in size from 1 to 48 bp,of which only two are common among the 23 total Pyraloidea moths that have data collected on the mitogenome(one is located between tRNAGln and ND2 with variation change in length and a limited sequence conservation,and the other is located between tRNASer(UCN) and ND1 with a conserved 6 bp motif ’ATACTA’).The AT-rich region of 331 bp in the genome is comprised of non-repetitive sequences but contains an ATAGN motif followed by a poly-T stretch of 17 bp,a microsatellite-like(TA)11 element preceded by an ATTTA motif,and a poly-A stretch upstream tRNAMet.These conserved structures identified in the AT-rich region are presented in all of the sequenced Pyraloidea species.We provide a mitogenome-based phylogeny of Pyraloidea species,in which L.sticticalis shares close ancestry to Ostrinia species with substantial evidence.Our phylogenetic analyses strongly divide Crambidae into two sister lineages.The mitogenome dataset also supports the basal split between Pyraustinae and Spilomelinae.更多还原