Targeted drug delivery systems for drug-resistant bacterial biofilms

【作者】 胡海燕；

【机构】 中山大学药学院；

【摘要】 Bacteria biofilms were more resistant to antibacterial agents than plank tonic bacteria due to the presence of extracellular polysaccharide substances(EPS),decreased metabolic rate and altered phenotype.Biofilms have become the overwhelming reason for antibiotic resistance.In our studies,the antibiofilm actions of different compounds on several Gram-negative and Gram-positive bacteria were investigated.We also compared the antibiofilm effect between the conventional three-drug strategy and the united novel use of antibiofilm agents and antibiotics on H.pylori biofilms.The results showed that the latter was more effective on antibiofilm action.Furthermore,we developed various types of lipid polymer nanoparticles which aim at enhancing the eradication rate of H.pylori by disrupting biofilms as well as inhibiting the adherence and colonization of H.pylori to target cells.The outer lipid layer of nanoparticles is often designed to contain antibiofilm agents.The inner core contains firstline antibiotics and anti-adhesion polymers.When encountering biofilms,the outer lipid layer including antibiofilm agents could destroy the biofilms by dissolving their EPS.Then the free planktonic bacteria exposed from the disrupted biofilms could be easily eradicated by antibiotics and anti-adhesion polymer by inhibiting adherence of H.pylori and the direct antimicrobial effect.更多还原

【Abstract】 Bacteria biofilms were more resistant to antibacterial agents than plank tonic bacteria due to the presence of extracellular polysaccharide substances(EPS),decreased metabolic rate and altered phenotype.Biofilms have become the overwhelming reason for antibiotic resistance.In our studies,the antibiofilm actions of different compounds on several Gram-negative and Gram-positive bacteria were investigated.We also compared the antibiofilm effect between the conventional three-drug strategy and the united novel use of antibiofilm agents and antibiotics on H.pylori biofilms.The results showed that the latter was more effective on antibiofilm action.Furthermore,we developed various types of lipid polymer nanoparticles which aim at enhancing the eradication rate of H.pylori by disrupting biofilms as well as inhibiting the adherence and colonization of H.pylori to target cells.The outer lipid layer of nanoparticles is often designed to contain antibiofilm agents.The inner core contains firstline antibiotics and anti-adhesion polymers.When encountering biofilms,the outer lipid layer including antibiofilm agents could destroy the biofilms by dissolving their EPS.Then the free planktonic bacteria exposed from the disrupted biofilms could be easily eradicated by antibiotics and anti-adhesion polymer by inhibiting adherence of H.pylori and the direct antimicrobial effect.更多还原