【作者】 Barry Kamira； 史磊磊； 范立民； 张聪； 郑尧； 孟顺龙； 胡庚东； 炳旭文； 陈家长； 徐跑；

【Author】 Barry Kamira;Lei Lei Shi;Li Min Fan;Cong Zhang;Yao Zheng;Shun Long Meng;Geng Dong Hu;Xu Wen Bing;Jia Zhang Chen;Pao Xu;Wuxi Fisheries College, Nanjing Agricultural University;Freshwater Fisheries Research Centre, Chinese Academy of Fishery Sciences/Fishery Eco-Environment Monitoring Center of Lower Reaches of Yangtze River, Ministry of Agriculture/Key Open Laboratory for Genetic Breeding and Aquaculture Biology,Ministry of Agriculture;

【机构】 南京农业大学无锡渔业学院； 中国水产科学研究院淡水渔业研究中心/农业部长江下游渔业生态环境监测中心/农业部淡水鱼类遗传育种和养殖生物学重点开放实验室；

【摘要】 为了解养殖水环境细菌的活性、多样性、分布和作用,使用生物过滤器的富集产生甲烷的需氧和厌氧氨氧化细菌来评估它们对水质的影响。使用PCR扩增和DGGE技术进行DNA测序评估16S rRNA基因的表达。五种主要的氨氧化细菌菌株-克隆;通过系统发育分析分离和鉴定KB.13,KB.15,KB.16,KB.17和KB.18-作为与甲基杆菌属,斯坦尼亚属,亚硝化单胞菌属和Heliorestis属密切相关的环境样品。由此发现的甲基氨氧化微生物表明涉及电子供体和碳源的生化途径,并且所有菌株在淡水水产养殖系统中都起作用。主成分分析表明,温度升至约40℃会显着影响环境特征-特别是DO,TN和NH₄⁺-N-并直接或间接地影响微生物群落。更多还原

【Abstract】 In this study, we used microbial enrichment of bio-filters to assess their effect on water quality. Profiles of ammonia-oxidizing bacterial communities generated using nested PCR methods and DGGE were used to assess the expression of 16 S rRNA genes using DNA sequencing. Five dominant ammonia-oxidizing bacterial strains–clones; KB.13, KB.15, KB.16, KB.17 and KB.18—were isolated and identified by phylogenetic analysis as environmental samples closely related to genera Methylobacillus, Stanieria, Nitrosomonas, and Heliorestis. Environmental parameters including TN; COD; NH⁴⁺-N; NO^2-N; NO^3-N, and DO assessed varied in the ranges in the different tanks. Principal component analysis revealed that these water quality parameters significantly influenced the ammonia oxidizing microbial community composition. Temperature rises to about 40 °C significantly affected environmental characteristics—especially DO, TN and NH4+-N—and directly or indirectly affected the microbial communities.更多还原