【作者】 张石云； 宋超； 董欣悦； 范立民； 张聪； 裘丽萍； 陈家长；

【Author】 ZHANG Shiyun;SONG Chao;DONG Xinyue;FAN Liming;ZHANG Cong;QIU Liping;CHEN Jiazhang;Wuxi fisheries college, Nanjing Agricultural university;

【机构】 南京农业大学无锡渔业学院；

【摘要】 以SCT 1084—2006《磺胺类药物水产养殖使用规范》为依据,制作无(NS)、低(LS)、中(MS))和高(HS)剂量磺胺甲噁唑(Sulfamethoxazole,简称SMZ)的饲料,通过8周的养殖试验(前4周投喂SMZ饲料,后4周投喂无SMZ饲料)、16s高通量测序技术和生物信息学分析来研究养殖过程中不同剂量SMZ的使用对养殖水体细菌群落结构的影响。第2、4、6和8周时运用16s rRNA和生物信息学测定分析水体细菌多样性。研究显示:1、不同剂量SMZ投喂下对养殖水体细菌的物种丰度和物种多样性有不同程度的降低影响,其中MS和HS效果显著;停止投喂SMZ饲料后显著性差异消失、差异程度降低;2、不同剂量SMZ投喂时均改变了细菌群落结构,其中NS群落和LS群落相似,与MS和HS群落差异大;3、SMZ的抑菌和细菌耐药作用显著改变水体菌群从门至属的物种组成,SMZ主要诱导了变形菌门中β-变形菌纲中某些参与氮循环相关的细菌(如:Candidatus_Branchiomonas和12up),抑制了一些致病菌(如:拟杆菌门的黄杆菌等)。研究结果表明,不同剂量饲料拌喂SMZ不仅会在不同程度上显著改变养殖水体细菌群落结构且有一定的延续性;此外,在SMZ的选择压力下一些与脱氮相关的菌成为明显的优势种,可能会对养殖池塘的氮循环有潜在的影响。更多还原

【Abstract】 Based on the specification for the Application of Sulfonamides in Aquaculture(SCT 1084--2006), without(NS), low(LS), medium(MS) and high(HS) doses of Sulfamethoxazole(SMZ) fodder were made. The effects of different doses of SMZ on the bacterial community structure of aquaculture water were studied through a 8 weeks of culture experiment( the first 4 weeks were SMZ diet and the last 4 weeks were no SMZ feed), 16 s high-throughput sequencing technology and bioinformatics analysis. Bacterial diversity in water was determined at week 2, 4, 6 and 8 weeks using 16 s r RNA and bioinformatics.The research showed that: 1. Feeding with different doses of SMZ were different degrees to reduced the species abundance and species diversity of cultured water bacteria, among which the effects of MS and HS were significant; After SMZ feed were stopped, the significant difference disappeared and the degrees of difference decreased. 2. The bacterial community structure were changed when SMZ were fed at different doses, among which the NS community and LS community were similar, with a large difference from MS and HS community. 3. The bacteriostatic and bacterial resistance of SMZ were significantly changed the species composition of water bacteria from phylum to genus. SMZ mainly induces some bacteria related to nitrogen cycle in the β-proteobacteria of proteobacteria(e.g., Candidatus_Branchiomonas and 12 up), and inhibits some pathogenic bacteria(e.g., flavobacterium of bacteroides). The results showed that feed with different doses of SMZ mixed could not only significantly change the community structure of bacteria in the aquaculture water, but also have some continuity. In addition, under the selective pressure of SMZ, some bacteria related to denitrification became obvious dominant species, which may have a potential impact on the nitrogen cycle in aquaculture water.更多还原